Cloning and Characterization of a Novel cDNA Encoding Late Embryogenesis-Abundant Protein 5 Like (LEA-5) Gene from Cara Cara Navel Orange Fruit (Citrus sinensis Osbeck)

查看全文 作　　者：TAO Neng-guo YE Jun-li XU Juan DENG Xiu-[1]xin 机构地区：[1]National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular Breeding, Huazhong Agricultural University, Wuhan 430070, P.R.China 出　　处：《Agricultural Sciences in China》索引2006年第5卷第4期,共5页 基　　金：the National Natural Science Foundation of China (30300241, 30471201). 摘　　要：LEA5 gene was postulated related with both stress and hormone responses. In an attempt to find genes exclusively expressed during fruit ripening of Cara Cara navel orange, a novel cDNA clone encoding late embryogenesis-abundant protein 5 like gene (CitLEA5-1) was obtained. It was 582 bp in length, containing 97 deduced amino acids. Compared with the stress-induced LEA5 from leaves of Citrus sinensis, CitLEA5-1 had a shorter 3′ untranslated region (UTR). Semi- quantitative RT-PCR analysis revealed that CitLEA5-1 was transcriptional regulated during fruit ripening of Cara Cara navel orange. 关 键 词：柑桔 克隆技术 胚胎发生 蛋白质 基因