Porous Calcium Phosphate Ceramic Scaffolds for Tissue Engineering

查看全文 作　　者：L Di [1]Silvio;N [2]Gurav;J [3]Merry;R [3]Sambrook 机构地区：[1]Biomaterials & Biomimetics Group, King's College London, UK;[2]Institute of Orthopaedics, University College London,UK;[3]Dytech, Corporation Ltd. Dronfield, Sheffield S18 1LS, UK 出　　处：《Journal of Wuhan University of Technology(Materials Science)》索引2005年第20卷第B12期,共3页 摘　　要：This study examined the biological response of two porous calcium phosphate ceramics, hydroxyapatite (HA) and hydroxyapaptite/β-tricalcium phosphate (HA/β-TCP) scaffolds. Three different cell types, a human osteoblastic cell line (HOS), primary human osteoblasts (HOB) and human mesenchymal stem cells (MSCs), were used to examine biocompatibility and osteoinductive capacity. The experimental results showed both materials were highly biocompatible and proliferation was significantly greater on pure HA (P< 0.01), with a peak in proliferation at day 7. Protein levels were significantly higher (P<0.05) than the control Thermanox (TMX(tm)) for both test materials. Osteoinduction of MSCs was observed on both test materials, with cells seeded on HA/β-TCP showing greater alkaline phosphatase activity compared to HA alone, indicating an enhancement in osteoinductive property. Both materials show good potential for use as tissue engineered scaffolds. 关 键 词：磷酸钙陶瓷骨 组织工程 间充质干细胞 骨移植