胰岛素样生长因子I降低黄体颗粒细胞凋亡,与胚胎破碎和妊娠率相关

查看全文 作　　者：Bencomo E.;Pérez R.;ArteagaM.-F.;A. [1]Palumbo;马超 机构地区：[1]Centro de Asistencia a la Reproducció n Humana de Canarias, C/Doctor Zamenhof 14, E- 38204 La Laguna, Tenerife, Spain,Dr. 出　　处：《世界核心医学期刊文摘(妇产科学分册)》索引2006年第2卷第6期,共2页 摘　　要：Objective: To correlate apoptosis of cultured human granulosa- lutein cells (GL cells) with the outcome of IVF (embryo fragmentation and pregnancy rate) and to study the effect of insulin and insulin- like growth factor I (IGF- I) on apoptosis. Design: In vitro assays. Setting: University laboratory and private IVF center. Patient(s): Eighty- one women undergoing IVF. Intervention(s): Purified human GL cells from pooled follicles were cultured for 48 hours in serum- free media with or without insulin and IGF- I. Cumulus cells and mural GL cells were evaluated separately. Main Outcome Measure(s): Detection of apoptosis by using caspACE FITC- VAD- FMK, a fluorescent in situ marker for activated caspases; embryo fragmentation; and pregnancy. Result(s): Age younger than 38 years and successful pregnancy were associated with less apoptosis (33.0% ± 17.2% vs. 43.2% ± 18.0% and 30.2% ± 14.0% vs. 40.4% ± 19.5% , respectively). There was a linear correlation between embryo fragmentation and GL cell apoptosis. Insulin- like growth factor I decreased apoptosis in a dose- dependent fashion. A statistically significant effect (17% decrease) was reached at a dose of 10 nM. Insulin (10 nM) caused a small (8% ) decrease in apoptosis, but this effect did not reach statistical significance. Cumulus cells consistently had < 3% apoptosis. Conclusion(s): [1] Apoptosis of cultured GL cells may be associated with IVF outcome and ovarian reserve and [2] IGF- I decreases apoptosis of cultured GL cells. 关 键 词：胰岛素样生长因子Ⅰ(IGF-Ⅰ) 颗粒细胞凋亡 妊娠率 胚胎 黄体 无血清培养基 荧光标记物 剂量依赖性 体外受精 体外测定