减少卵子老化对于补救性卵母细胞单精子显微注射结局的影响

查看全文 作　　者：Nagy [1]Z.P.;Rienzi L.F.;Ubaldi F.M.;朱亮 机构地区：[1]Reproductive Biology Associates, 1150 Lake Hearn Drive,Atlanta, GA 30342, United States Dr 出　　处：《世界核心医学期刊文摘(妇产科学分册)》索引2006年第2卷第9期,共2页 摘　　要：Objective: To evaluate the results of a novel protocol that allows to rescue IVF unfertilized oocytes by intracytoplasmic sperm injection (ICSI).Design: Prospective clinical trial.Setting: Private reproductive medical center.Patient(s): Thirty patients undergoing IVF.Intervention(s): Controlled ovarian stimulation (COS), conventional IVF, rescue ICSI, embryo culture, and embryo transfer.Main Outcome Measure(s): Identification of unfertilized IVF oocytes 6 hours after insemination and fertilization, and developmental rates of those oocytes after rescue microinjection, as well implantation and pregnancy rates (PR).Result(s): All oocytes (392) from 30 patients were inseminated with standard IVF 3 hours after ovum pick- up.Polar body (PB) status was checked at decumulation and rechecked 3 hours later.Eighty- two oocytes were fertilized after IVF alone and 184 nonactivated oocytes (failed fertilization) were rescue microinjected and 166 of them fertilized (20 patients).Cleavage stage on day 2 was significantly more advanced and embryo grade was higher after standard IVF fertilization than after rescue ICSI.Eight of the 30 embryos transferred were implanted in the IVF- only patients (27% )- and 8 of 68 embryos in the rescue ICSI patients (12% ).Conclusion(s): Rescue ICSI of unfertilized IVF oocytes 6 hours after insemination (9 hours after egg retrieval) can provide normal fertilization, embryo development, and pregnancy; however, corresponding outcome parameters tend to be impaired in comparison to the standard IVF fertilization results. 关 键 词：卵母细胞单精子显微注射 卵子老化 补救性 补救ICSI 未受精卵 胚胎移植 IVF 卵子受精