Differentially Expression of Tual,a Tubulin-encoding Gene,during Flowering of Tea Plant Camellia sinensis (L.) O.Kuntze Using cDNA Amplified Fragment Length Polymorphism Technique

查看全文 作　　者：Wan-Ping [1,2]FANG;Chang-Jun [1]JIANG;Mei [1]YU;Ai-Hua [1]YE;Zhao-Xia [1,3]WANG

机构地区：[1]Key Laboratory of Tea Biochemistry and Biotechnology, Ministry of Agriculture, Anhui Agricultural University, Hefei 230036. China;[2]College Of Horticulture, Nanjing Agriculture University, Nanjing 210095, China;[3]Auhui Institute of Education. Hefei 230036, China

出　　处：《Acta Biochimica et Biophysica Sinica》索引2006年第38卷第9期,共10页

基　　金：This work was supported by a grant from the Natural Science Foundation of Anhui Province (No.050410102) 摘　　要：The complementary DNA (cDNA) amplified fragment length polymorphism technique wasused to isolate transcript-derived fragments corresponding to genes involved in the flowering of tea plant.Comparative sequence analysis of an approximately 300 bp differential fragment amplified by primer combi-nation E_(11)M_(11) revealed 80%-84% similarity to the corresponding part of an α-tubulin gene of other species.The complete cDNA sequence of this α-tubulin was cloned by the rapid amplification of cDNA ends technique;its full length is 1537 bp and contains an open reading frame of 450 amino acid residues with two N-glycosylation sites and four protein kinase C phosphorylation sites.The deduced amino acid sequences didshow significant homology to the α-tubulin from other plants that has been reported to be a pollen-specificprotein and could be correlated with plant cytoplasm-nucleus-interacted male sterility.We named this completecDNA Tual.The nucleotide and amino acid sequence data of Tual have been recorded in the GenBanksequence database with the accession No.DQ340766.This Tual gene was cloned into the pET-32aexpression system and expressed in Escherichia coli BL21trxB(DE3).The molecular weight of expressedprotein was deduced to be approximately 49 kDa.Western blot analysis was used to identify the temporalexpression of Tual in tea plant.Further studies of the effect of Tual protein on pollen tube growth indicatedthe Tual solution obviously promoted the growth of tea pollen tube. 关键词：微管蛋白基因编码山茶花基因多态性

相关文献

参考文献（30）
引证文献（9）
耦合文献（33）

参考文献(30)

引证文献(9)

耦合文献(33)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Differentially Expression of Tual,a Tubulin-encoding Gene,during Flowering of Tea Plant Camellia sinensis (L.) O.Kuntze Using cDNA Amplified Fragment Length Polymorphism Technique

参考文献(30)

引证文献(9)

耦合文献(33)

用户提示