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The study of migration of bone mesenchymal stem cells transplanted in intervertebral discs of rabbits and expression of exogenous gene

查看全文 作　　者：Jintang Wang Hong Zhang Yingang Zhang Xiaowei Zhang Meng [1]Li 高影响力作者 机构地区：[1]Department of Orthopaedics, the First Affiliated Hospital, Medical School, Xi'an Jiaotong University, Xi'an 710061,China高影响力机构 出　　处：《Journal of Nanjing Medical University》索引2006年第20卷第5期,共5页高影响力期刊 基　　金：The Study of Differentiation of Bone Mesenchymal Stem Cells Transplanted in Intervertebral Disc and Expression of ExogenousGene(30400163) 摘　　要：Objective: To explore the survival and migration of bone mesenchymal stem cells transplantated in intervertebral disc of rabbits and expression of the exogenic genes. Methods: Thirty-two rabbits were used, A randomized block design was used and discs in the same rabbit were one block,the lumbar discs from L2-3 to L5-6 were randomly divided into blank group, saline group, cell transplantation groupⅠ and cell transplantation groupⅡ. The fluorescence microscopy was used to determine the fluorescence of the maker protein GFP and DNA-PCR was used to analyze the copies of DNA of neomycin-resistant gene at 1, 3, 6, months after transplantation. Results: There was fluorescence in cell transplantation group Ⅰand Ⅱ and none in blank group, saline group at 1, 3, 6 months after transplantation. In cell transplantation groups,the fluorescent distribution was more scatter with time, but no significant difference between cell groups Ⅰand Ⅱ. The test of neomycin resistant gene expressed in cell transplantation group ⅠandⅡ and quantitative analysis showed that there was no significant difference between the cell groups Ⅰand Ⅱ(P > 0.05). Conclusion: The transplanted bone mesenchymal stem cells can survive, migrate and the transfer genes can express efficiently, it suggests that the BMSC therapy may be effective to prevent and treat intervertebral disc degeneration. 关 键 词：骨髓间质干细胞 椎骨 外源基因 治疗
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