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Stimulation of melanogenesis by scoparone in B16 melanoma cells

查看全文 作　　者：Jeong-yeh [1]YANG;Jeung-[2]hyunKOO;Young-[2]gilSONG;Kang-beom [3]KWON;Ju-hyung [4]LEE;Hee-sook [5]SOHN;Byung-hyun [6]PARK;Eun-chung [1]JHEE;Jin-woo [7,8]PARK 高影响力作者 机构地区：[1]Department of Biochemistry,Dental School,Chonbuk National University,Jeonju 561-756,Korea;[2]Department of Biochemistry Medical School,Chonbuk National University,Jeonju 561-756,Korea;[3]Department of Physiology,School of Oriental Medicine,Won-Kwang University,Iksan 570-749,Korea;[4]Department of Preventive Medicine,Medical School;[5]Department of Food Science and Human Nutrition,College of Home Economy,Chonbuk National University,Jeonju 561-756,Korea;[6]Department of Biochemistry,Medical School,Chonbuk National University,Jeonju 561-756,Korea Institute for Healthcare Technology Development,Chonbuk National University,Jeonju 561-756,Korea;[7]Department of Biochemistry,Medical School,Chonbuk National University,Jeonju 561-756,Korea;[8]Institute for Healthcare Technology Development,Chonbuk National University,Jeonju 561-756,Korea高影响力机构 出　　处：《Acta Pharmacologica Sinica》索引2006年第27卷第11期,共7页高影响力期刊 基　　金：This work was supported by the Korea Research Foundation Grant funded by the Korean Government(MOEHRD)(the Center for Healthcare Technology Development,Chonbuk National University,Jeonju 561-756,Republic of Korea). 摘　　要：Aim:The effect of coumarin derivatives on melanogenesis was investigated inB16 murine melanoma cells.Methods:Melanin content and tyrosinase activitywere analyzed spectrophotometrically.The expression of tyrosinase,tyrosinase-related protein-1(TRP-1)and tyrosinase-related protein-2(TRP-2)were measuredeither by reverse transcription-polymerase chain reaction(RT-PCR)or Westernblot.Results:Among the coumarin derivatives studied,scoparone(6,7-dimethoxycoumarin)was the most potent;the 6-or 7-methoxy group was found tobe essential for the stimulation of melanogenesis.The melanin content was greatlyincreased by scoparone in a dose-dependent manner;there was no cytotoxicity atthe effective concentrations.Scoparone increased enzyme activity as well asprotein and mRNA expression of tyrosinase.In addition,mRNA of TRP-1 andTRP-2 were also increased after treatment with scoparone.H-89,an inhibitor ofprotein kinase A(PKA),completely inhibited the scoparone-induced increase ofmelanogenesis and the tyrosinase protein.Conclusion:These results suggestthat scoparone-induced stimulation of melanogenesis is likely to occur at thetranscriptional level of melanogenesis-related enzymes through PKA signaling. 关 键 词：黑素生成 东喘宁 黑素瘤 刺激作用
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