Transpositional behaviour of the Ds element in the Ac/Ds system in rice

查看全文 作　　者：LIU [1]Fang;ZHANG [1]XiangQian;ZHANG [1]ZeMin;CHEN [1]ZhaoGui;ZHU [1]HaiTao;WANG [2]Jiang;ZHANG [2]JingLiu;ZHANG [1]GuiQuan

机构地区：[1]College of Agriculture, South China Agricultural University, Guangzhou 510642, China;[2]National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Science, Chinese Academy of Sciences, Shanghai 200032, China

出　　处：《Chinese Science Bulletin》索引2007年第52卷第20期,共8页

基　　金：Supported by National Basic Research Program of China (Grant No. G19990116) 摘　　要：Insertional mutagenesis based on maize Activator/Dissociator (Ac/Ds) transposons is becoming a ma- jor approach used to produce a saturated mutant collection in rice. In this research, Ds-T-DNA trans- formed homozygotes were crossed with Ac-T-DNA transformed homozygotes in order to establish an Ac/Ds transposon system in rice. The successive investigation of Ds transposition from F1 to F5 gen- erations indicated that the frequencies of germinal transposition increased over successive genera- tions and reached 54.2% in F3 generation. The Ds transposition pattern revealed that a Ds transposition induced an approximately 170-bp deletion of T-DNA sequence and another Ds transposition carried a 272-bp T-DNA sequence. Using thermal asymmetric interlaced PCR (TAIL-PCR), some flanking se- quences of the Ds element were amplified. Analyses of 17 Ds-flanking sequences showed that five Ds were inserted into gene regions. The Ds could transpose not only to the linked sites but also to the unlinked sites. The frequency of inter-chromosomal transposition of Ds was 33.3%. 关键词：置换行为稻米激活剂幼芽

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Transpositional behaviour of the Ds element in the Ac/Ds system in rice

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