Effect of notoginsenoside R1 on hepatic microcirculation disturbance induced by gut ischemia and reperfusion

查看全文 作　　者：Wei-Xing [1,2]Chen;Fang Wang, Yu-Ying Liu, Qing-Jiang Zeng, Kai Sun, Xin Xue, Xiang Li, Ji-Ying Yang, Li-Hua An,Bai-He Hu, Jin-Hui Yang, Chuan-She Wang, Zhi-Xin Li, Lian-Yi Liu, Yan Li, Jun Zheng, Fu-Long Liao, Dong Han,Jing-Yu Fan, Jing-Yan Han Fang [1]Wang;Yu-Ying [1]Liu;Qing-Jiang [1]Zeng;Kai [1]Sun;Xin [1]Xue;Xiang [1]Li;Ji-Ying [1,2]Yang;Li-Hua [1]An;Bai-He [1]Hu;Jin-Hui [1]Yang;Chuan-She [1,3]Wang;Zhi-Xin [1,3]Li;Lian-Yi [1]Liu;Yan [1]Li;Jun [1,2]Zheng;Fu-Long [1]Liao;Dong [1]Han;Jing-Yu [1,3]Fan;Jing-Yan [1]Han

机构地区：[1]Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing 100083, China;[2]Department of Pharmacology, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;[3]Department of Integrated Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing 100083, China

出　　处：《World Journal of Gastroenterology》索引2008年第14卷第1期,共9页

基　　金：Supported by Tianjin Tasly Group, Tianjin, China 摘　　要：AIM: To assess the effect of notoginsenoside R1 on hepatic microcirculatory disturbance induced by gut ischemia/reperfusion (I/R) in mice.METHODS: The superior mesenteric artery (SMA) of C57/BL mice was ligated for 15 min to induce gut ischemia followed by 30-min reperfusion. In another set of experiments, R1 was continuously infused (10 mg/kg per hour) from 10 min before I/R until the end of the investigation to study the influence of R1 on hepatic microcirculatory disturbance induced by gut I/R. Hepatic microcirculation was observed by inverted microscopy, and the vascular diameter, red blood cell (RBC) velocity and sinusoid perfusion were estimated. Leukocyte rolling and adhesion were observed under a laser confocal microscope. Thirty and 60 min after reperfusion, lactate dehydrogenase (LDH), alanine aminotransferase (ALT) and aspartate transaminase (AST) in peripheral blood were determined. The expression of adhesion molecules CD11b/CD18 in neutrophils and tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6) and monocyte chemotactic protein-1 (MCP-1) in plasma were evaluated by flow cytometry. E-selectin and intercellular adhesion molecule-1 (ICAM-1) in hepatic tissue were examined by immunofluorescence. RESULTS: After gut I/R, the diameters of terminal portal venules and central veins, RBC velocity and the number of perfused sinusoids were decreased, while the leukocyte rolling and adhesion, the expression of E-selectin in hepatic vessels and CD18 in neutrophils, IL-6, MCP-1, LDH, ALT and AST were increased. R1 treatment attenuated these alterations except for IL-6 and MCP-1.CONCLUSION: R1 prevents I/R-induced hepatic microcirculation disturbance and hepatocyte injury. The effect of R1 is related to its inhibition of leukocyte rolling and adhesion by inhibiting the expression of E-selectin in endothelium and CD18 in neutrophils. 关键词：缺血/再灌注肝微循环紊乱肝损伤肝功能

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Effect of notoginsenoside R1 on hepatic microcirculation disturbance induced by gut ischemia and reperfusion

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