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Establishment and Characterization of a Cell Based Artificial Antigen-Presenting Cell for Expansion and Activation of CD8^+ T Cells Ex Vivo

查看全文 作　　者：Weijuan [1]Gong;Mingchun [1]Ji;Zhengfeng [1]Cao;Liheng [1]Wang;Yayun [1]Qian;Maozhi [2]Hu;Li [1]Qian;Xingyuan [1]Pan 高影响力作者 机构地区：[1]Department of Immunology, School of Medicine, Yangzhou University, Yangzhou 225001, China;[2]Testing Centre, Yangzhou University, Yangzhou 225001, China高影响力机构 出　　处：《Cellular & Molecular Immunology》索引2008年第5卷第1期,共7页高影响力期刊 基　　金：the National Natural Science Foundation of China(No.30400399,No.30671917);the Natural Science Fund of Jiangsu Province(BK2004404) ;the Natural Science Fund of the Educational Committee of Jiangsu Province(04KJB320162) in China. 摘　　要：Artificial antigen-presenting cells are expected to stimulate the expansion and acquisition of optimal therapeutic features of T cells before infusion. Here CD32 that binds to a crystallizable fragment of IgG monoclonal antibody was genetically expressed on human K562 leukemia cells to provide a ligand for T-cell receptor. CD86 and 4-1BBL,which are ligands of co-stimulating receptors of CD28 and 4-1BB,respectively,were also expressed on K562 cells. Then we accomplished the artificial antigen-presenting cells by coupling K32/CD86/4-1BBL cell with OKT3 monoclonal antibody against CD3,named K32/CD86/4-1BBL/OKT3 cells. These artificial modified cells had the abilities of inducing CD8+ T cell activation,promoting CD8+ T cell proliferation,division,and long-term growth,inhibiting CD8+ T cell apoptosis,and enhancing CD8+ T cell secretion of IFN-γ and perforin. Furthermore,antigen-specific cytotoxic T lymphocytes could be retained in the culture stimulated with K32/CD86/4-1BBL/OKT3 cells at least within 28 days. This approach was robust,simple,reproducible and economical for expansion and activation of CD8+ T cells and may have important therapeutic implications for adoptive immunotherapy. 关 键 词：细胞 人工神经系统 活化作用 抗原体
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