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Transgenic tobacco plants expressing synthetic Cry1Ac and Cry1Ie genes are more toxic to cotton bollworm than those containing one gene

查看全文 作　　者：LIAN [1]Yun;JIA [1]ZhiWei;HE [2]KangLai;LIU [3]Yundun;SONG [2]FuPing;WANG [1]BaoMin;WANG [3]GuoYing 高影响力作者 机构地区：[1]State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100094, China;[2]State Key Laboratory for Biology of Plant Diseases and Insect Pests, institute of Plant Protection, Chinese Academy of Agriculture Sciences, Beijing 100094, China;[3]Institute of Crop Sciences, Chinese Academy of Agricalture Sciences, Beijing 100081, China高影响力机构 出　　处：《Chinese Science Bulletin》索引2008年第53卷第9期,共7页高影响力期刊 基　　金：Supported by the National Plant Transformation and Industrialization Program of China (Grant No. JY03-A-13) 摘　　要：Transgenic tobacco plants carrying Cry1Ac, Cry1Ie or both genes were obtained. In the leaves of transgenic plants carrying both genes, the contents of Cry1Ac and Cry1Ie proteins were 0.173% and 0.131% of the total proteins, respectively. Cry1Ac protein content was 0.182% and Cry1Ie protein con- tent was 0.124% of the total proteins in the leaves of transgenic plants containing only one Bt gene. Fresh leaves of transgenic tobacco and wild-type plants were used for the insect bioassay against wild-type and Cry1Ac-resistant cotton bollworm (Helicoverpa armigera). The bioassay results showed that transgenic plants carrying both genes were significantly more toxic to wild-type and Cry1Ac-resistant cotton bollworm than those carrying Cry1Ac or Cry1Ie alone. This study indicates that the higher toxicity of transgenic tobacco plants carrying both genes is caused by the cooperative function of both Bt proteins, thus providing a potential way to delay the development of insect resis- tance to transgenic crops. 关 键 词：锕 烟草 中毒 棉花螟蛉
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