Overexpression of Sweet Pepper Glycerol-3-Phosphate Acyltransferase Gene Enhanced Thermotolerance of Photosynthetic Apparatus in Transgenic Tobacco

查看全文 作　　者：Kun Yan Na Chen Yan-Yan Qu Xin-Chun Dong Qing-Wei Meng Shi-Jie [1]Zhao

机构地区：[1]College of Life Sciences, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an 271018, China

出　　处：《Journal of Integrative Plant Biology》索引2008年第50卷第5期,共9页

基　　金：the Early Stage of China Key Development Project for BasicResearch (CB116208);the National Natural Science Foundation of China(30471053);the Shandong Provincial Natural Science Foundation ofChina (Y2007D50). 摘　　要：In order to investigate the relationship between the lipid composition in thylakoid membrane and thermostability of pho-tosynthetic apparatus,tobacco transformed with sweet pepper sense glycerol-3-phosphate acyltransferase (GPAT) gene were used to analyze the lipid composition in thylakoid membrane,the net photosynthetic rate and chlorophyll fluorescence parameters under high temperature stress. The results showed that the saturated extent of monogalactosyldiacylglycerol (MGDG),sulfoquinovosyldiacylglycerol,digalactosyldiacylglycerol and phosphatidylglycerol in thylakoid membrane of transgenic tobacco T1 lines increased generally. Particularly,the saturated extent in MGDG increased obviously by 16.2% and 12.0% in T1-2 and T1-1,respectively. With stress temperature elevating,the maximum efficiency of photosystem II (PSII) photochemistry (Fv/Fm),actual photochemical efficiency of PSII in the light ( PSII) and net photosynthetic rate (Pn) of the two lines and wild type tobacco plants decreased gradually,but those parameters decreased much less in transgenic plants. Even though the recovery process appeared differently in the donor and acceptor side of PSII in transgenic tobacco compared with wild-type plants,the entire capability of PSII recovered faster in transgenic tobacco,which was shown in the parameters of PI,Fv/Fm and PSII,as a result,the recovery of Pn was accelerated. Conclusively,we proposed that the increase in saturated extent of thylakoid membrane lipids in transgenic plants enhanced the stability of photosynthetic apparatus under high temperature stress. 关键词：磷酸转移酶类囊体隔膜光合作用烟草

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Overexpression of Sweet Pepper Glycerol-3-Phosphate Acyltransferase Gene Enhanced Thermotolerance of Photosynthetic Apparatus in Transgenic Tobacco

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