Cloning and Characterization of Largemouth Bass(Micropterus salmoides) Myostatin Encoding Gene and Its Promoter

查看全文 作　　者：LI Shengjie BAI Junjie WANG [1]Lin

机构地区：[1]Key Laboratory of Tropical and Subtropical Fish Breeding and Cultivation of CAFS, Pearl RiverFisheries Research Institute, CAFS, Guangzhou 510380, P. R. China

出　　处：《Journal of Ocean University of China》索引2008年第7卷第3期,共7页

摘　　要：Myostatin or GDF-8,a member of the transforming growth factor-β(TGF-β) superfamily,has been demonstrated to be a negative regulator of skeletal muscle mass in mammals. In the present study,we obtained a 5.64 kb sequence of myostatin encoding gene and its promoter from largemouth bass(Micropterus salmoides) . The myostatin encoding gene consisted of three exons(488 bp,371 bp and 1779 bp,respectively) and two introns(390 bp and 855 bp,respectively) . The intron-exon boundaries were conservative in comparison with those of mammalian myostatin encoding genes,whereas the size of introns was smaller than that of mammals. Sequence analysis of 1.569 kb of the largemouth bass myostatin gene promoter region revealed that it contained two TATA boxes,one CAAT box and nine putative E-boxes. Putative muscle growth response elements for myocyte enhancer factor 2(MEF2) ,serum response factor(SRF) ,activator protein 1(AP1) ,etc.,and muscle-specific Mt binding site(MTBF) were also detected. Some of the transcription factor binding sites were conserved among five teleost species. This information will be useful for studying the transcriptional regulation of myostatin in fish. 关键词：克隆技术黑鲈鱼类基因编码

相关文献

参考文献（12）
引证文献（4）
耦合文献（0）

参考文献(12)

引证文献(4)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Cloning and Characterization of Largemouth Bass(Micropterus salmoides) Myostatin Encoding Gene and Its Promoter

参考文献(12)

引证文献(4)

用户提示