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Lactobacilli inhibit interleukin-8 production induced by Helicobacter pylori lipopolysaccharide-activated Toll-like receptor 4

查看全文 作　　者：Chao Zhou Feng-Zhen Ma Xue-Jie Deng Hong Yuan Hong-Sheng [1]Ma 高影响力作者 机构地区：[1]Department of Gastroenterology, West China Hospital of Sichuan University, Chengdu 610041, Sichuan Province, China高影响力机构 出　　处：《World Journal of Gastroenterology》索引2008年第14卷第32期,共6页高影响力期刊 基　　金：Basic Research Project of Science and Technology Offi ce of Sichuan Province, No. 04JY029-090-1 摘　　要：AIM: To investigate the effect of Lactobacillus bulgaricus (LBG) on the Toll-like receptor 4 (TLR4) pathway and interleukin-8 (IL-8) production in SGC-7901 cells treated with Helicobacter pyloriSydney strain 1 lipopolysaccharide (HpyloriSS1-LPS). METHODS: SGC-7901 cells were treated with Hpylori SS1-LPS in the presence or absence of pretreatment for 1 h with viable LBG or supernatant recovered from LBG culture MRS broth (LBG-S). Cellular lysates were prepared for Western blot with anti-TLR4, anti-transforming growth factor β-activated kinase 1 (TAK1), anti-phospho-TAK1, anti-nuclear factor κB (NF-κB), anti-p38 mitogen-activated protein kinase (p38MAPK), and anti-phospho-p38MAPK antibodies. The amount of IL-8 in cell culture medium was measured by ELISA. RESULTS: H pylori SS1-LPS up-regulated the expression of TLR4, stimulated the phosphorylation of TAK1, subsequently enhanced the activation of NF- κB and the phosphorylation of p38MAPK in a time- dependent manner, leading to augmentation of IL-8 production in SGC-7901 cells. Viable LBG or LBG-S pretreatment attenuated the expression of TLR4, inhibited the phosphorylation of TAK1 and p38MAPK, prevented the activation of NF-κB, and consequently blocked IL-8 production.CONCLUSION: H pylori SS1-LPS induces IL-8 production through activating TLR4 signaling in SGC-7901 cells and viable LBG or LBG-S prevents H pyloriSS1-LPS-mediated IL-8 production via inhibition of the TLR4 pathway. 关 键 词：乳酸菌 脂多糖 白介素-8 临床医学
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