Torque teno virus:Its prevalence and isotypes in North India

查看全文 作　　者：Mohammad [1]Irshad;Shiwani [1]Singh;Khushboo [2]Irshad;Sanjay Kumar [3]Agarwal;Yogendra Kumar [4]Joshi

机构地区：[1]Clinical Biochemistry Division,Department of Laboratory Medicine,All India Institute of Medical Sciences,New Delhi-110029,India;[2]Department of Biochemistry,All India Institute of Medical Sciences,New Delhi-110029,India;[3]Department of Nephrology,All India Institute of Medical Sciences,New Delhi-110029,India;[4]Department of Gastroenterology and Human Nutrition,All India Institute of Medical Sciences,New Delhi-110029,India

出　　处：《World Journal of Gastroenterology》索引2008年第14卷第39期,共8页

基　　金：Indian Council of Medical Research (ICMR),New Delhi-110049 for financial grant 摘　　要：AIM: To investigate the prevalence and genotype dis-tribution of Torque teno virus (TTV) in patients with different liver diseases and chronic renal failure treated at a referral hospital in North India. METHODS: Whereas prevalence of TTV was based on amplification of conserved region of ORF2 of TTV genome,the genotyping of TTV was carried out using restriction fragment length polymorphism (RFLP) procedure on the N22 region of ORF1. RESULTS: TTV-DNA was detected in 137 of 513 (26.7%) patients with liver diseases and 38 of 65 (58.5%) patients with chronic renal failure. TTV was also detected in 27% of healthy controls. The sequence analysis of the PCR product from 10 randomly selected cases failed to show a significant sequence divergence when compared with that of the TRM1 isolate of TTV genotype 1. The results of genotyping in 55 randomly selected patients showed the presence of genotype 1 (G1) in 53 (96.4%) and genotype 2 (G2) in 2 cases (3.6%),respectively. Other genotypes were not identified in this patient subgroup,suggesting that G1 is predominant in this area. The results of genotyping by RFLP were also supported by phylogenetic tree analysis,where G1 was found to be the major genotype. CONCLUSION: These results indicate that TTV is moderately present in Indian patients,with G1 to be the major genotype in North India. The pathogenicity and etiological role of TTV in different diseases is still a question mark and warrant further studies. 关键词：病毒感染基因型多态性印度

相关文献

参考文献（32）
引证文献（6）
耦合文献（2）

参考文献(32)

引证文献(6)

耦合文献(2)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Torque teno virus:Its prevalence and isotypes in North India

参考文献(32)

引证文献(6)

耦合文献(2)

用户提示