Identification and characterization of genotype A and D recombinant hepatitis B virus from Indian chronic HBV isolates

查看全文 作　　者：Ranjit [1,2]Chauhan;Syed Naqui [3]Kazim;Manoj [1]Kumar;Jayashree [2]Bhattacharjee;Narayanasamy [4]Krishnamoorthy;Shiv Kumar [1]Sarin

机构地区：[1]Department of Gastroenterology and Advanced Center for Liver Diseases,G.B.Pant Hospital;[2]Department of Biochemistry,Lady Hardinge Medical College;[3]Centre for Interdisciplinary Research in Basic Sciences;[4]The Centre for Genomic Application

出　　处：《World Journal of Gastroenterology》索引2008年第14卷第40期,共9页

基　　金：Indian Council of Medical Research-Advanced Center for Liver Diseases Project (ICMR-ACLD) 摘　　要：AIM: To confirm the presence of recombination,full-length hepatitis B virus (HBV) from chronic patients was sequenced and analyzed.METHODS: Full-length HBV genomes from 12 patients were amplified and sequenced in an automated sequencer.Phylogenetic analysis was carried out on full-length,Core and preS2/Surface regions using MEGA software.SimPlot Boot Scanning and amino acid sequence analysis were performed for confirmation of recombination.RESULTS: Eight patients were infected with genotype D strain; one patient with genotype A and three patients had genotype A and D recombination; two of them had cirrhosis and one had hepatocellular carcinoma.Phylogenetic analysis of core and preS2/surface regions separately showed evidence of genotype A and D recombination.The breakpoints of recombination were found to be at the start of preS2 and at the end of surface coding regions.CONCLUSION: We identified and characterized recombinant A and D genotype HBV in hepatitis B surface antigen (HBsAg)-positive patients. 关键词：乙型肝炎遗传型进化变化重组

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Identification and characterization of genotype A and D recombinant hepatitis B virus from Indian chronic HBV isolates

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