Microcalorimetric investigation of effect of berberine alkaloids from Coptis chinensis Franch on intestinal diagnostic flora growth

查看全文 作　　者：YAN [1]Dan;WEI [1]Li;XIAO [1]XiaoHe;ZHOU [1]DanLei;HAN [2]YuMei

机构地区：[1]Institute of Chinese Medicine, 302 Hospital of People's Liberation Army, Beijing 100039, China;[2]Chongqing Institute of Technology, Chongqing 400050, China

出　　处：《Chinese Science Bulletin》索引2009年第54卷第3期,共5页

基　　金：Supported by the National Natural Science Foundation of China (Grant Nos. 30625042 and 30873385);Key Projects of National Science & Technology Pillar Program of China in the 11th Five-Year Period (Grant No. 2007BAI40B05) 摘　　要：The inhibitory effect of three berberine alkaloids (BAs) from Coptis chinensis Franch, a traditional Chinese medicinal (TCM) herb, on Bifidobacterium adolescentis growth was investigated by micro- calorimetry. The power-time curves of B. adolescentis with and without BAs were acquired, meanwhile the extent and duration of inhibitory effect on the metabolism were evaluated by the growth rate con- stant (k), half inhibitory ratio (IC50), maximum heat-output power (Pmax), peak time of maximum heat-output power (tp) and total heat production (Qt). k, Pmax and Qt decreased, and tp was prolonged with the increase of BAs concentration. The IC50 of BAs is 806 μg/mL for berberine, 341 μg/mL for cop- tisine and 236 μg/mL for palmatine. The sequence of antimicrobial activity of BAs is berberine < cop- tisine < palmatine. Combined with previous studies, it could be shown that the sequences of antim- icrobial activity of BAs on both Bacillus shigae and Escherichia coli are berberine > coptisine > pal- matine. The structure-function relationship of BAs indicates that the functional group methylenedioxy or methoxyl at C2 and C3 might be the major group inducing the activities of BAs on E. coli and B. adolescentis. Meanwhile, the substituent groups at C2, C3, C9 and C10 almost have equal effect on B. shigae. 关键词：黄连素生物碱细菌大肠杆菌抑制效应

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Microcalorimetric investigation of effect of berberine alkaloids from Coptis chinensis Franch on intestinal diagnostic flora growth

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