Prevalence of vacA, cagA and babA2 genes in Cuban Helicobacter pylori isolates

查看全文 作　　者：Lino E [1]Torres;Karelia [2]Melián;Arlenis [1]Moreno;Jordis [2]Alonso;Carlos A [3]Sabatier;Mayrín [1]Hernández;Ludisleydis [1]Bermúdez;Boris L [1]Rodríguez

机构地区：[1]Department of Microbiology and Immunology, Biotechnology Division, National Centre for Scientif ic Research, Ciudad de La Habana, Cuba;[2]Department of Gastroenterology, Medical and Chirurgic Research Centre (CIMEQ), Ciudad de La Habana, Cuba;[3]Anatomopathology Department, Medical and Chirurgic Research Centre (CIMEQ), Ciudad de La Habana, Cuba

出　　处：《World Journal of Gastroenterology》索引2009年第15卷第2期,共7页

基　　金：Supported by The National Centre for Scientific Research of Cuba, No. 220207 摘　　要：AIM: To investigate the prevalence of vacuolating cytotoxin (vacA), cytotoxin associated gene A (cagA) and blood adhesion binding antigen (babA2) genotypes of Helicobacter pylori (H pylori) isolates from Cuban dyspeptic patients. METHODS: DNA was extracted from H pylori-positive cultures taken from 130 dyspeptic patients. Genotyping was performed by PCR, using specifi c primers for vacA (s1, s2, m1, m2), cagA and babA2 genes. Endoscopic observations and histological examinations were used to determine patient pathologies. RESULTS: vacA alleles s1 , s2 , m1 and m2 were detected in 96 (73.8%), 34 (26.2%), 75 (57.7%) and 52 isolates (40%), respectively, while the cagA gene was detected in 95 isolates (73.2%). One hundred and seven isolates (82.3%) were babA2-positive. A signif icant correlation was observed between vacAs1m1 and cagA and between vacAs1m1 and babA2 genotypes (P < 0.001 and P < 0.05, respectively) and between babA2 genotype and cagA status (P < 0.05); but, no correlation was observed between vacAs1 and babA2 genotypes. Eighty fi ve (65.4%) and 73 (56.2%) strains were type 1 (vacAs1-cagA-positive) and 'triple- positive' (vacAs1-cagA-babA2-positive), respectively, and their presence was significantly associated with duodenal ulcer (P < 0.01 and P < 0.001, respectively). CONCLUSION: The distribution of the main virulence factors in the Cuban strains in this study resembled that of the Western-type strains, and the more virulent H pylori isolates were signifi cantly associated with duodenal ulcer, ulcer disease being the worst pathology observed in the group studied. 关键词：古巴消化系统疾病基因 VACA CAGA babA

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Prevalence of vacA, cagA and babA2 genes in Cuban Helicobacter pylori isolates

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