Highly sensitive detection of melamine based on reversed phase liquid chromatography mass spectrometry

查看全文 作　　者：WU [1,2]QingQing;FAN [1]KeXin;SHA [1]Wei;RUAN [1]HongQiang;ZENG [1]Rong;SHIEH [1,2]ChiaHui

机构地区：[1]Key Laboratory of Systems Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China;[2]Shanghai KeyTag Bioscience Inc., Shanghai 200233, China

出　　处：《Chinese Science Bulletin》索引2009年第54卷第5期,共6页

基　　金：Supported by the National Natural Science Foundation of China (Grant No. 30425021);National High Technology Research and Development Program of China (Grant No. 2007AA02Z334) 摘　　要：In this work, we developed a highly sensitive method to detect melamine based on reversed phase liquid chromatography mass spectrometry. A mass spectrometry compatible ion pair, heptafluorobu-tyric acid(HFBA), was used to separate melamine by reversed phase liquid chromatography prior to electrospray mass spectrometry. The incorporation of isotope internal standard and multiple reaction monitoring improved the accuracy and linearity of quantification. Based on this strategy, the method limit of quantification was 0.1 ng/g. The limits of quantification were 8 ng/g for liquid milk and 15 ng/g for dry milk powder. This method provided a reproducible and stable approach to sensitive detection and quantification of melamine. 关键词：反相流相色谱质谱分析法离子对多反应监测模式三聚氰胺

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Highly sensitive detection of melamine based on reversed phase liquid chromatography mass spectrometry

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