Docosahexaenoic acid suppresses arachidonic acid-induced proliferation of LS-174T human colon carcinoma cells

查看全文 作　　者：Piet [1,2]Habbel;Karsten H [1,2]Weylandt;Katja [1,2]Lichopoj;Johannes [1,2]Nowak;Martin [1]Purschke;Jing-Dong [1]Wang;Cheng-Wei [1]He;Daniel C [1]Baumgart;Jing X [1]Kang

机构地区：[1]Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, United States;[2]Department of Medicine, Division of Gastroenterology and Hepatology, Charité Medical Center-Virchow-Hospital, Medical School of the Humboldt-University of Berlin, 13344 Berlin, Germany

出　　处：《World Journal of Gastroenterology》索引2009年第15卷第9期,共6页

基　　金：Supported by Grants from the German National Academic Foundation (to P.H.);from the American Cancer Society (RSG-03-140-01-CNE);the NIH (NIH R01 113605) (both to J.X.K.);the German Research Foundation (DFG);a Charité Research Grant (both to K.H.W.) 摘　　要：AIM:To investigate the impact of arachidonic acid (AA) and docosahexaenoic acid (DHA) and their combination on colon cancer cell growth.METHODS:The LS-174T colon cancer cell line was used to study the role of the prostaglandin precursor AA and the omega-3 polyunsaturated fatty acid DHA on cell growth. Cell viability was assessed in XTT assays. For analysis of cell cycle and cell death,flow cytometry and DAPI staining were applied. Expression of cyclooxygenase-2 (COX-2),p21 and bcl-2 in cells incubated with AA or DHA was examined by real-time RT-PCR. Prostaglandin E2 (PGE2) generation in the presence of AA and DHA was measured using a PGE2-ELISA.RESULTS:AA increased cell growth,whereas DHAreduced viability of LS 174T cells in a time-and dose-dependent manner. Furthermore,DHA down-regulated mRNA of bcl-2 and up-regulated p21. Interestingly,DHA was able to suppress AA-induced cell proliferation and significantly lowered AA-derived PGE2 formation. DHA also down-regulated COX-2 expression. In addition to the effect on PGE2 formation,DHA directly reduced PGE2-induced cell proliferation in a dose-dependent manner. CONCLUSION:These results suggest that DHA can inhibit the pro-proliferative effect of abundant AA or PGE2. 关键词：二十二碳六烯酸人结肠癌细胞花生四烯酸诱导扫描仪 LS 酶联免疫吸附试验前列腺素E2 酸抑制

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Docosahexaenoic acid suppresses arachidonic acid-induced proliferation of LS-174T human colon carcinoma cells

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