维普中文期刊产品整合服务

Identification and Analysis of Genetic Diversity Structure Within Pisum Genus Based on Microsatellite Markers

查看全文 作　　者：ZONG Xu-[1]xiao;Rebecca [2]Ford;Robert R [3]Redden;GUAN Jian-[1]ping;WANG Shu-[1]min 高影响力作者 机构地区：[1]Institute of Crop Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R. China;[2]BioMarka, Faculty of Land and Food Resources, University of Melbourne, Melbourne 3010, Australia;[3]Australian Temperate Field Crops Collection, Grains Innovation Park, Department of Primary Industries, Private Bag 260, Horsham 3401, Australia高影响力机构 出　　处：《Agricultural Sciences in China》索引2009年第8卷第3期,共11页高影响力期刊 基　　金：supported by the National Key Technologies R&D Program of China (2006BAD13B05,2006BAD02B08);the National Program for Crop Germplasm Protection of China (NB07-2130135-(25-30)-13);the National Science and Technology Plat-form R&D Program of China (2005DKA21001-6);the China-ACIAR Project (ACIAR:CS1/2000/035). 摘　　要：To assesse the genetic diversity among wild and cultivated accessions of 8 taxonomic groups in 2 species, and 5 subspecies under Pisum genus, and to analyze population structure and their genetic relationships among various groups of taxonomy, the study tried to verify the fitness of traditionally botanical taxonomic system under Pisum genus and to provide essential information for the exploration and utilization of wild relatives of pea genetic resources. 197 Pisum accessions from 62 counties of 5 continents were employed for SSR analysis using 21 polymorphic primer pairs in this study. Except for cultivated field pea Pisum sativum ssp. sativum var. sativum (94 genotypes), also included were wild relative genotypes that were classified as belonging to P. fulvum, P. sativum ssp.abyssinicum, P. sativum ssp. asiaticum, P. sativum ssp. transcaucasicum, P. sativum ssp. elatius var. elatius, P. sativum ssp. elatius var. pumilio and P. sativum ssp. sativum var. arvense (103 genotypes). The PCA analyses and 3-dimension PCA graphs were conducted and drawn by NTSYSpc 2.2d statistical package. Nei78 genetic distances among groups of genetic resources were calculated, and cluster analysis using UPGMA method was carried out by using Popgene V1.32 statistical package, the dendrogram was drawn by MEGA3.1 statistical package. Allelic statistics were carried out by Popgene V1.32. The significance test between groups of genotypes was carried out by Fstat V2.9.3.2 statistical package. 104 polymorphic bands were amplified using 21 SSR primer pairs with unambiguous unique polymorphic bands. 4.95 alleles were detected by each SSR primer pair in average, of which 65.56% were effective alleles for diversity. PSAD270, PSAC58, PSAA18, PSAC75, PSAA175 and PSAB72 were the most effective SSR pairs. SSR alleles were uniformly distributed among botanical taxon units under Pisum genus, but significant difference appeared in most pairwise comparisons for genetic diversity between taxon unit based groups of genetic resources. Genetic diversity level of wild species P. fulvum was much lower than the cultivated species P. sativum. Under species P. sativum, P. sativum ssp. sativum var. sativum and P. sativum ssp. asiaticum were the highest in gentic diversity, followed by P. sativum ssp. elatius var. elatius and P. sativum ssp. transcaucasicum, P. sativum ssp. elatius var. pumilio, P. sativum ssp. sativum var. arvense and P. sativum ssp. abyssinicum were the lowest. Four gene pool clusters were detected under Pisum genus by using PCA analysis. Gene pool 'fulvum' mainly consisted of wild species Pisum fulvum, gene pool 'abyssinicum' mainly consisted of P. sativum ssp. abyssinicum, and gene pool 'arvense' mainly consisted of P. sativum ssp. sativum var. arvense. While gene pool 'sativum' were composed by 5 botanical taxon units, they are P. sativum ssp. asiaticum, P. sativum ssp. elatius var. elatius, P. sativum ssp. transcaucasicum, P. sativum ssp. elatius var. pumilio and P. sativum ssp. sativum var. sativum. 'sativum' gene pool constructed the primary gene pool of cultivated genetic resources; 'fulvum' gene pool, 'abyssinicum' gene pool and 'arvense' gene pool together constructed the secondary gene pool of cultivated genetic resources. Pairwise Nei78 genetic distance among botanical taxon based groups of pea genetic resources ranged from 7.531 to 35.956, 3 large clustergroups were identified based on the UPGMA dendrogram. Group I equals to 'sativum' and 'arvense' gene pools, Group II equals to 'abyssinicum' gene pool, and Group III equals to 'fulvum' gene pool. The UPGMA clustering results generally supporting the PCA clusting results. There were significant differences among most botanical groups under Pisum genus, with clear separation of four gene pools for genetic diversity structure. The research results partially support the traditional botanical taxonomy under Pisum genus, and pointed out its advantage and shortcoming. In order to broaden the genetic bases of pea varieties, the genetic potentials in the four gene pools should be thoroughly exploited. 关 键 词：遗传多样性分析 人口结构 豌豆 微卫星标记 聚类分析方法 统计软件包 过磷酸钙 SSR引物
相关文献

参考文献(10)

引证文献(5)

网站首页 | 关于我们 | 联系我们 | 产品服务 | 客服中心 | 广告服务 | 版权声明 | 网站联盟 | 友情链接 | 售卡网点

版权所有© 渝B2-20050021-1　渝公网安备 50019002500403号　违法和不良信息举报中心

互联网出版许可证 新出网证(渝)字10号 全国400电话 - 免长途话费