Increment of antioxidase activity of transgenic tobacco with betaine aldehyde dehydrogenase

查看全文 作　　者：LUO [1]Ailing;LIU [1]Jaoyao;MA [2]Deqin;WANG [3]Xuechen;LIANG [1]Zheng

机构地区：[1]Photosynthesis Research Center,Institute of Botany,Chinese Academy of Sciences,Beijing 100093,China;[2]Institute of Microbiology,Chinese Academy of Sciences,Beijing 100080,China;[3]Chinese Agricultural University,Beijing 100094,China

出　　处：《Chinese Science Bulletin》索引2001年第46卷第6期,共4页

基　　金：the Stale Key BasicResearch Developmental Program of China (Grant No. (11999011700) and the Natural Science Foundation of Shandong (Gram No. Y9X02037). 摘　　要：Superoxide dismutase (SOD) activity in the leaves of transgenic tobacco plants with betaine aldehyde dehydrogenase (BADH) gene was about 36% higher than that in the control plants (parent plants), activities of per-oxidase (POD) and catalase (Cat) increased by about 62% and 88% respectively. Activities of ascorbate peroxidase (AsSPOD), dehydroascorbate redutase (DAsAR) and glu-tathione reductase (GR) in ascorbate-glutothion pathway located at chloroplasts increased by 67.7%, 47.9% and 38.8% respectively. These results indicated that the H2O2 produced by SOD catalyzing superoxide anion radicals (O2-) could be fully decomposed, and could not derive to form the strongest toxicant radicals · OH. This is the first report to elucidate quantitatively that the activities of two kinds of antioxidative enzymes decomposed radicals and active oxygen were matched. Photoinhibition tolerant capacity of the transgenic tobacco plants was 35% higher than that in the parent plants. Increment of photoinhibition tolerant 关键词：betaind ALDEHYDE DEHYDROGENASE gene TOBACCO antioxi- dative ENZYMES activities.

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Increment of antioxidase activity of transgenic tobacco with betaine aldehyde dehydrogenase

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