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Sex-related gene and sex identification of Crested Ibis Nipponia nippon (Ciconiifor-mes: Threskiornithidae)

查看全文 作　　者：LI [1]Ming;DING [1]Changqing;WEI [1]Fuwen;MENG [2]Shijie;XI [3]Yongmei;LU [3]Baozhong 高影响力作者 机构地区：[1]Institute of Zoology,Chinese Academy of Sciences,Beijing 100080,China;[2]Department of Biology,Northwest University,Xi’an 710060,China;[3]Shaanxi Crested Ibis Conservation Station,Yangxian 723300,China高影响力机构 出　　处：《Chinese Science Bulletin》索引2001年第46卷第8期,共3页高影响力期刊 基　　金：the National Natural ScienceFoundation of China (Grant No. 39870098), the Science and Technology Innovation Program of the Chinese Academy of Sciences (CAS) (Grant No. KSCX2-1-03) and the Young Scientist Group of the CAS. 摘　　要：The Crested Ibis (Nipponia nippon) is a critical endangered species of the world. At present, a reintroduction program will be conducted to save this species essentially. However, because the Crested Ibise is a sexual alike bird, it is very difficult to identify the sex from the morphological character. In order to identify the sex easily and select the right individuals for captive breeding and reintroduction, the sex related gene on W chromosome was amplified and the sex of three Crested Ibises were also identified in the present study. The 262bp fragment was also sequenced, and we found that there were 13 different nucleotide sites with 2.25 of transition/transversion based on the comparison with that of the Oriental White Stork. The sequence will also provide a theoretic base for further designing specific primer for sex related gene in the Crested Ibis. 关 键 词：Crested IBIS Nipponia NIPPON SEX-RELATED GENE SEX identification.
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