Thymidine kinase gene mutation leads to reduced virulence of pseudorabies virus

查看全文 作　　者：Pan, [1]ZS;Zhang, [1]CY;Ding, [1]JH;Min, [1]P

机构地区：[1]Wuhan Univ, Coll Life Sci, Inst Virol, Wuhan 430072, Peoples R China

出　　处：《Chinese Science Bulletin》索引2001年第46卷第23期,共4页

摘　　要：To explore correlation between the tk gene structure of pseudorabies virus (PRV) and its virulence, to study the effect of the gene mutation on PRV biological properties, and to investigate mechinism of reduced virulence, thymidine kinase (TK)-deficient mutant of pseudorabies virus strain Hubei (PRV HB) was isolated by selection for resistance to 5-bromodeoxyuridine. The tk genes of PRV HB and its TK mutant were cloned and sequenced. 1587 base pairs of the tk gene and flanking regions of wild-type (wt) virus were sequenced, which included an open reading frame (ORF) of 1098 bp encoding a protein of 366 amino acids. The ORF contained two 137-bp repeated sequences, which were connected by an adenosine. 1458 bp of the tk and flanking regions of TK- mutant were sequenced. Analysis of the tk gene sequence of TK mutant indicated that one of 137 bp repeated sequence and the connecting adenosine in the tk gene of the wt virus was deleted and a repeated sequence of 8 nucleotides (GCGCGCC) was inserted. All 关键词：PSEUDORABIES virus THYMIDINE kinase NUCLEOTIDE se- quence amino acid sequence TK mutant.

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Thymidine kinase gene mutation leads to reduced virulence of pseudorabies virus

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