Immunohistochem ical Study of T Lym phocyte Subsets onFrozen Substituted Plastic Em bedding Section of the BoneMarrow from Patients with Myelodysplastic Syndrom e andIts ClinicalIm plication

查看全文 作　　者：LIU Shangqing , LIU Xiufang , ZOU Ping , SONG Shanjun , WANG Bianming Institute of Hematology, Xiehe Hospital, Tongji Medical University, Wuhan 430022 出　　处：《Journal of Huazhong University of Science and Technology(Medical Sciences)》索引1999年第19卷第3期,共5页 摘　　要：An immunohistochemical study of T lymphocyte subsets on frozen substituted plastic embedding bone marrow sections obtained from 10 patients with myelodysplastic syndrome (MDS) was presented. The results of qualitative and quantitative immunohistochemical analysis are as follows: (1) Labile antigens of T lymphocytes were well preserved, thus allowing analysis of distribution of T lymphocyte subsets in situ ; (2) the average number of T 3, T 4 and T 8 lymphocyte of the diffuse infiltrate was about 2 %, 0.4 %, 0.5 %, respectively, of all nucleated cells in bone marrow, and T 4/T 8 of T cells were below 1.0 in patients with MDS; (3) there were cases of RAS showing T lymphocyte aggregation in bone marrow, but no patient exhibited progressive refractory anemia with excess of blasts(RAEB) and RAEB in transformation (RAEBT). These findings indicated that the immunological abnormalities are of importance in the evaluation of pathogenesis and prognosis of MDS. 关 键 词：immunohistochemistry PLASTIC embedding of bone marrow distribution of T cell SUBSETS in situ MYELODYSPLASTIC syndrome