Expression of neurofilament gene in spinal motoneurons during neural regeneration

查看全文 作　　者：Ruifa Mi;Xiangqun Shi;Shuhong Liu;Changman Zhou;Jiazheng Wang;Ming Fan

机构地区：[1]Acad Mil Med Sci, Inst Basic Med Sci, Beijing 100850, Peoples R China;[2]Beijing Med Coll PLA, Beijing 100071, Peoples R China;

出　　处：《Chinese Science Bulletin》索引1999年第44卷第3期,共5页

摘　　要：The technique in situ hybridization was used to measure the levels of light (NF-L),medium(NF-M) and heavy (NF-H) neurofilament protein subunits mRNA in L_4-6 spinal motoneurons in adult rat during regeneration following a unilateral crush of the sciatic nerve.It was found that the hybridization signals of each neurofilament subunit mRNA were dramatically decreased in spinal motoneurons postaxotomy by light microscopy. The hybridization signals of NF-L and NF-M mRNA were located in cytoplasm of neurons, whereas NF-H mRNA was found in both nucleus and cytoplasm of neurons. lmage analysis showed that the encoding levels of mRNA for each of neurofilament subunit mRNA reduced on the 3rd d and returned to control levels on the 28th d following the lesion. The relative levels of mRNA coding for each neurofilament subunit were significantly different. The lowest level of NF-L mRNA was observed at 5 d postaxotomy, and that of NF-M, NF-H mRNA on the 7th and 10th d after injury. Moreover,the levels of HF-M and 关键词：NEUROFILAMENT mRNA neural REGENERATION SPINAL MOTONEURONS in SITU HYBRIDIZATION rat.

相关文献

参考文献（10）
引证文献（0）
耦合文献（6）

参考文献(10)

耦合文献(6)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Expression of neurofilament gene in spinal motoneurons during neural regeneration

参考文献(10)

耦合文献(6)

用户提示