Physical Location of the Rice Pi-5(t), Glh and RTSV Genes by ISH of BAC Clones

查看全文 作　　者：Yan Huimin, Song Yunchun, Li Lijia, Bi Xuezhi, Fu Binying(College of Life Sciences, Wuhan University, Wuhan 430072, China)

出　　处：《Wuhan University Journal of Natural Sciences》索引1998年第3卷第2期,共5页

摘　　要：Mapplng of low of slngle-copy sequences on plant chromosomes has proven dirricult because ofvery low frequency or slgnal detectlon. Rlce BAC Ilbrary ls belng used wldely ln rlce genome research due tolts distlnctlve advantages over other llbrary systems. In tkls study, two blotln-Iabeled rice BAC clones closelylinked to a rlce blast reslstance, green Ieaf hopper reslstance and tungro spherlcal virus resistance gene,Pi-5(t),Glh, RTSV, were in situ hybrldlred to rlce ckromosomes. They were located on the long arm andshort arm of ckromosome 4 with FL value of 40% and 100% respectively-The rrequency of signal detectionreacked 46. 8% and 59- 2%- The slgnal location were conslstent wltk the selectlve marker on rice saturatedm0Iecular map. The results demonstrated the advautages to Iocate BAC cIones to ckromosomes by in sho hybrldization and will racilitate tke rlce low of slngle-copy gene Iocation by using the BAC library. 关键词：BAC CLONE BIOTIN labeling in SITU HYBRIDIZATION

相关文献

参考文献（13）
引证文献（25）
耦合文献（0）

参考文献(13)

引证文献(25)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Physical Location of the Rice Pi-5(t), Glh and RTSV Genes by ISH of BAC Clones

参考文献(13)

引证文献(25)

用户提示