Down-Regulated Expression of RACK1 Gene by RNA Interference Enhances Drought Tolerance in Rice

查看全文 作　　者：LI Da-[1,2]hong;LIU [1]Hui;YANG Yan-[1]li;ZHEN Ping-[1]ping;LIANG Jian-[1]sheng

机构地区：[1]Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, China;[2]Department of Agronomy and Forestry, Huanghuai College, Zhumadian 463000, China

出　　处：《Rice science》索引2009年第16卷第1期,共7页

基　　金：supported by the National Natural Science Foundation of China (Grant No. 30571120);the National High Technology Research and Development Program of China (Grant No.2008AA10Z120);the Research Fund for the Doctoral Program of Higher Education, China 摘　　要：The receptor for activated C-kinase 1 (RACK1) is a highly conserved scaffold protein with versatile functions, and plays important roles in the regulation of plant growth and development. Transgenic rice plants, in which the expression of RACK1 gene was inhibited by RNA interference (RNAi), were studied to elucidate the possible functions of RACK1 in responses to drought stress in rice. Real-time PCR analysis showed that the expression of RACK1 in transgenic rice plants was inhibited by more than 50%. The tolerance to drought stress of the transgenic rice plants was higher as compared with the non-transgenic rice plants. The peroxidation of membrane and the production of malondialdehyde were significantly lower, and the superoxide dismutase activity in transgenic rice plants was significantly higher than those in non-trangenic rice plants. It is suggested that RACK1 negatively regulated the redox system-related tolerance to drought stress of rice plants. 关键词：转基因水稻植株 RNA干扰基因抑制超氧化物歧化酶活性抗旱性干旱胁迫氧化还原系统 PCR分析

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Down-Regulated Expression of RACK1 Gene by RNA Interference Enhances Drought Tolerance in Rice

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