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DNA Fingerprinting of Ninety Wild Strains of Agaricus from China

查看全文 作  者:CHEN [1]Meiyuan;LIAO [1]Jianhua;WANG [2]Bo;LI [1]Hongrong;LU [1]Zhenghui;GUO [1]Zhongjie;CAI [1]Danfeng;WANG [1]Zesheng 高影响力作者 机构地区:[1]Fujian Mushroom R & D Station, Edible Fungi Institute of Fujian Academy of Agricultural Sciences,Fuzhou, Fujian 350014, China;[2]Soil and Fertilizer Institute of Sichuan Academy of AgriculturalSciences, Chengdu, Sichuan 610000, China高影响力机构 出  处:《食用菌学报》索引2009年第16卷第1期,共4页高影响力期刊 基  金:Supported by National Fund of Natural Sciences(No.30671456,30771503);Key Science & Technology Programsof Fujian Province(No.2006S1001);Fujian Fund of Natural Sciences(No.B0620004);Key S&T InnovationItems of Fujian Academy of Agricultural Sciences(2007) 摘  要:Ninety wild strains of Agaricus collected in China,including 44 strains preliminarily identified as Agaricus bisporus by isozyme electrophoresis,were studied using SRAP and ISSR techniques.Eighteen specific SRAP bands and 12 specific ISSR bands were scored,and a cluster dendrogram was constructed.The 90 wild strains of Agaricus were separated into two major groups,the first consisting of wild strains of A.bisporus and the second consisting of other Agaricus strains.Forty-one wild strains of A.bisporus collected in Sichuan and Tibet formed four groups according to the geographical location of the collection area.White A.bisporus strains from Xinjiang and Tibet(Xinjiang Wild,AgX04 and AgX042)exhibited unique band patterns resulting in lower similarity coefficient values(10%~13%)when compared with brown or light-brown A.bisporus strains. 关 键 词:双孢蘑菇 栽培技术 DNA 野生蘑菇
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