Enrichment and hydrogen production by marine anaerobic hydrogen-producing microflora

查看全文 作　　者：CAI [1,2]JinLing;WANG [1,3]GuangCe;LI [4]YanChuan;ZHU [3]DaLing;PAN [3]GuangHua

机构地区：[1]Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;[2]Graduate University of Chinese Academy of Sciences, Beijing 100049, China;[3]Key Laboratory of Marine Resources and Chemistry of Tianjin, Research Center for Algal Bioenergy and Environmental Effect, College of Marine Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China;[4]Biotechnology Research Center, Kunming University of Science and Technology, Kunming 650224, China

出　　处：《Chinese Science Bulletin》索引2009年第54卷第15期,共6页

基　　金：Supported by the National High Technology Research and Development Program of China (Grant No. 2006AA05Z112) 摘　　要：Acid, alkali, heat-shock, KNO3 and control pretreatment methods applied to anaerobic sludge were evaluated for their ability to selectively enrich the marine hydrogen-producing mixed microflora. Sea-water culture medium was used as the substrate. The hydrogen yield of pretreated microflora was higher than that of the un-pretreated control (P < 0.05). Among the pretreatment methods studied, heat-shock pretreatment yielded the greatest hydrogen production, which was 14.6 times that of the control. When the effect of initial pH on hydrogen production of heat-shock pretreated samples was studied, hydrogen was produced over the entire pH range (pH 4 - 10). The hydrogen yield peaked at initial pH 8 (79 mL/g sucrose) and then steadily decreased as the initial pH increased. Sucrose con-sumption was high at neutral initial pH. During the process of hydrogen production, pH decreased gradually, which indicated that the acquired microflora consisted of acidogenic bacteria. 关键词：生产过程菌群组成厌氧污泥产氢海洋初始PH值富集预处理

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Enrichment and hydrogen production by marine anaerobic hydrogen-producing microflora

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