Peak compression technique in high-performance liquid chromatography

查看全文 作　　者：WEI YuXia WANG Lin XIAO ShengYuan QING Hong ZHU Yong HU GaoFei DENG [1]YuLin

机构地区：[1]School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, China

出　　处：《Chinese Science Bulletin》索引2009年第54卷第16期,共5页

基　　金：Supported by the National Key Technology R&D Program in the 11th Five-year Plan (Grant No. 2007CB914100) 摘　　要：Peak compression technique based on the difference of the solute migration velocity in two different mobile phases was described theoretically and confirmed using benzaldehyde and 4-hydroxyquinoline (4-HQ) as model compounds. After peak compression, the peak compression factors (the ratio of peak width at half-height under non-compression and that under compression condition) of benzaldehyde and 4-HQ were 0.19 and 0.13, respectively. By this application of the peak compression technique to the mixture, both enhanced peak height and good separation were obtained in one run cycle. This peak compression technique was introduced to determine benzaldehyde from semicarbazide-sensitive amine oxidase-catalyzed enzymetic reaction in order to illustrate the applicability of this technique to the real sample. As a result, the peak was compressed effectively, and 4.94-fold, 19.3-fold and 5.74-fold enhancement in peak height, plate number and signal to noise ratio were also achieved, respectively. 关键词：压缩技术高效液相色谱模型化合物脲甲醛迁移速度羟基喹啉压缩条件运行周期

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Peak compression technique in high-performance liquid chromatography

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