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Oxidative stress and apoptosis of Carassius auratus lymphocytes induced by nonplanar (PCB153) and coplanar (PCB169) polychlorinated biphenyl congeners in vitro

查看全文 作　　者：ZHANG [1]Jianying;ZHANG [1,2]Hangjun;NI [1]Wanmin 高影响力作者 机构地区：[1]College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310028, China.;[2]College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China高影响力机构 出　　处：《Journal of Environmental Sciences》索引2009年第21卷第9期,共6页高影响力期刊 基　　金：supported by the Zhejiang Provincial Natural Science Foundation of China(No.Z506039);the National Key Basic Research and Development Program in China(No.2002CB410810) 摘　　要：Among all the 209 kinds of polychlorinated biphenyls(PCBs) congeners,nonplanar and coplanar PCB congeners have different levels of toxicity on mammal cells such as neuronal cells,but little is known about their toxicity on fish cells although PCB congeners usually have high bioaccumulation abilities in the detected fish bodies.This study showed that 2,2',4,4',5,5'-hexacholorbiphenyl(PCB153,nonplanar congener) and 3,3',4,4',5,5'-hexacholorbiphenyl(PCB169,coplanar congener) caused apoptosis on the isolated crucian carp(Carassius auratus) lymphocytes and the induced cytotoxicity was structure-dependent.According to the laser confocal microscope observations,apoptosis was clearly distinguished by condensation of nucleus,shrinkage and formation of apoptotic bodies.DNA fragmentation was detected by agarose gel electrophoresis.These typical morphological and biochemical characteristics indicate the occurrence of apoptosis on fish lymphocytes.According to the flow cytometry analysis,after the cells were exposed to 10μmol/L PCBs for 3 h,the apoptotic percentage induced by PCB153 was 23.41%,while that induced by PCB169 was even higher(31.03%) . Furthermore,incubating PCBs with fish lymphocytes enhanced levels of reactive oxygen species(ROS) and malondialdehyde(MDA) ,clearly indicating the presence of oxidative stress and lipid peroxidation.Our data also demonstrate that the different cytotoxic effects induced by coplanar and nonplanar PCBs were correlated with their structural characteristics and the coplanar congener was more cytotoxic than nonplanar congener.This study suggests that cytotoxicity mechanisms of the PCB congeners on fish lymphocytes depend on their planarity and chemical structures. 关 键 词：细胞凋亡 多氯联苯 淋巴细胞 氧化应激 非平面 诱导 体外 共面
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