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Expression of Bone-related Genes in Bone Marrow MSCs after Cyclic Mechanical Strain: Implications for Distraction Osteogenesis

查看全文 作　　者：Meng-chun [1,2]Qi;Shu-juan [1]Zou;Li-chi [1]Han;Hai-xiao [1]Zhou;Jing [1]Hu 高影响力作者 机构地区：[1]State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, China;[2]Department of Stomatology, North-China Coal Medical College, Tangshan, China高影响力机构 出　　处：《International Journal of Oral Science》索引2009年第1卷第3期,共8页高影响力期刊 基　　金：supported by grants from the National Nature Science Foundation of China (No. 30772454);Science and Technology Bureau of Sichuan Province (No. 2006z09-013) 摘　　要：Aim Understanding the response of mesenchymal stem cells (MSCs) to mechanical strain and their consequent gene expression patterns will broaden our knowledge of the mechanobiology of distraction osteogenesis. Methodology In this study, a single period of cyclic mechanical stretch (0.5 Hz, 2,000 με) was performed on rat bone marrow MSCs. Cellular proliferation and alkaline phosphatase (ALP) activity was examined. The mRNA expression of six bone-related genes (Ets-1, bFGF, IGF-Ⅱ, TGF-β, Cbfa1 and ALP) was detected using real-time quantitative RT-PCR. Results The results showed that mechanical strain can promote MSCs proliferation, increase ALP activity, and up-regulate the expression of these genes. A significant increase in Ets-1 expression was detected immediately after mechanical stimulation, but Cbfa1 expression became elevated later. The temporal expression pattern of ALP coincided perfectly with Cbfa1. Conclusion The results of this study suggest that mechanical strain may act as a stimulator to induce differentiation of MSCs into osteoblasts, and that these bone-related genes may play different roles in the response of MSCs to mechanical stimulation. 关 键 词：骨撑开牵引术 机械应变 间叶干细胞 基因表达
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