Isolation of marine benzo[a]pyrene-degrading Ochrobactrum sp. BAP5 and proteins characterization

查看全文 作　　者：WU [1]Yirui;HE [1]Tengteng;ZHONG [1]Mingqi;ZHANG [1]Yueling;LI [2]Enmin;HUANG [1]Tongwang;HU [1]Zhong

机构地区：[1]Department of Biology, Science College, Shantou University, Shantou 515063, China.;[2]Department of Biochemistry and Molecular Biology, Medical College, Shantou University, Shantou 515063, China

出　　处：《Journal of Environmental Sciences》索引2009年第21卷第10期,共6页

基　　金：supported by the National Natural Sci-ence Foundation of China (No 40206015, 30970106);the Fork Ying Tong Education Foundation (No 94002) ;the Science and Technology Project of Guangdong Province,China (No 2006A36502001, 2007A032600003) 摘　　要：A bacterial strain BAP5 with a relatively high degradation ability of benzo[a]pyrene (BaP) was isolated from marine sediments of Xiamen Western Sea, China and identified as Ochrobactrum sp. according to 16S rRNA gene sequence as well as Biolog microbial identification system. Strain BAP5 could grow in mineral salt medium with 50 mg/L of BaP and degrade about 20% BaP after 30 d of incubation. Ochrobactrum sp. BAP5 was able to utilize other polycyclic aromatic hydrocarbons (PAHs) (such as phenanthrene, pyrene and fluoranthene) as the sole carbon source and energy source, suggesting its potential application in PAHs bioremediation. The profile of total soluble protein from Ochrobactrum sp. BAP5 was also investigated. Some over- and special-expressed proteins of strain BAP5 when incubated with the presence of BaP were detected by two-dimensional polyacrylamide gel electrophoresis, and found to be related with PAHs metabolism, DNA translation, and energy production based on peptide fingerprint analysis through matrix-assisted laser desorption/ionization-time of flight mass spectrometry. 关键词：苯并[A]芘蛋白质鉴定海洋沉积物降解能力杆菌 RRNA基因序列电离飞行时间质谱多环芳烃

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Isolation of marine benzo[a]pyrene-degrading Ochrobactrum sp. BAP5 and proteins characterization

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