Knockdown of OLA1,a regulator of oxidative stress response,inhibits motility and invasion of breast cancer cells

查看全文 作　　者：Jia-wei [1]ZHANG;Valentina [2]RUBIO;Shu [1]ZHENG;Zheng-zheng [1,2]SHI

机构地区：[1]Cancer Institute (National Ministry of Education Key Laboratory of Cancer Prevention.and Intervention), the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China;[2]Department of Radiology, Methodist Hospital Researeh Institute, Houston, Texas 77030, USA

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》索引2009年第10卷第11期,共9页

基　　金：Project supported by the National Basic Research Program (973) of China (No. 2004CB518707) ;the Methodist Hospital Research Institute, USA 摘　　要：To explore the role of a novel Obg-like ATPase 1 (OLA1) in cancer metastasis,small interference RNA (siRNA) was used to knockdown the protein,and the cells were subjected to in vitro cell migration and invasion assays. Knockdown of OLA1 significantly inhibited cell migration and invasion in breast cancer cell line MDA-MB-231. The knockdown caused no changes in cell growth but affected ROS production. In wound-healing assays,decreased ROS in OLA1-knockdown cells were in situ associated with the cells’ decreased motile morphology. Further,treatment of N-acetylcysteine,a general ROS scavenger,blunted the motility and invasiveness of MDA-MB-231 cells,similar to the effect of OLA1-knockdown. These results suggest that knockdown of OLA1 inhibits breast cancer cell migration and invasion through a mechanism that involves the modulation of intracellular ROS levels. 关键词：乳腺癌细胞氧化应激反应侵袭运动小干涉RNA 细胞迁移 siRNA 肿瘤转移

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Knockdown of OLA1,a regulator of oxidative stress response,inhibits motility and invasion of breast cancer cells

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