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Accumulation of 1-deoxynojirimycin in silkworm,Bombyx mori L.

查看全文 作　　者：Hao [1,2]YIN;Xin-qin [1]SHI;Bo [1]SUN;Jing-jing [1]YE;Zu-an [1]DUAN;Xiao-ling [1]ZHOU;Wei-zheng [1]CUI;Xiao-feng [3]WU 高影响力作者 机构地区：[1]Key Laboratory of Agricultural Ecology and Environment, College of Forestry, Shandong Agricultural University, Taian 2 71018, China;[2]Institute of Sericultural Sciences of Siehuan Province, Nanchong 637000, China;[3]College of Animal Science, Zhejiang University, Hangzhou 310029, China高影响力机构 出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》索引2010年第11卷第4期,共6页高影响力期刊 摘　　要：1-deoxynojirimycin (1-DNJ) contents in the silkworm,Bombyx mori,at different developmental stages and tissues were investigated by using reverse-phase high-performance liquid chromatography. The 1-DNJ contents of silkworm larvae change significantly with their developmental stages. The male larvae showed higher accumulation efficiency of 1-DNJ than the females and also a significant variation was observed among the silkworm strains. The present results show that tissue distribution of 1-DNJ was significantly higher in blood,digestive juice,and alimentary canal,but no 1-DNJ was observed in the silkgland. Moreover,1-DNJ was not found in silkworms fed with artificial diet that does not contain mulberry leaf powder. This proves that silkworms obtain 1-DNJ from mulberry leaves; they could not synthesize 1-DNJ by themselves. The accumulation and excretion of 1-DNJ change periodically during the larval stage. There was no 1-DNJ in the newly-hatched larvae and 1-DNJ was mainly accumulated during the early and middle stages of every instar,while excreted at later stages of larval development. Further,it is possible to extract 1-DNJ from the larval feces and it is optimal to develop the 1-DNJ related products for diabetic auxiliary therapy. 关 键 词：关键词蚕 1-deoxynojirimycin (1-DNJ ) 累积 Bombyx mori
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