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Elevated expression of mature miR-21 and miR-155 in cancerous gastric tissues from Chinese patients with gastric cancer

查看全文 作　　者：Li [1,2]Liu;Qi [1]Chen;Rensheng [2]Lai;Xiaobin [2]Wu;Xiaoyu [3]Wu;Fukun [3]Liu;Guohua [4]Xu;Yong [1]Ji 高影响力作者 机构地区：[1]Atherosclerosis Research Center, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China;[2]Laboratory ot Cytology & Molecules/Department of Pathology, the Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Nanjing 210029, Jiangsu Province, China;[3]Department of Surgery, the Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Nanjing 210029, Jiangsu Province, China;[4]Department of Biology, Jiangsu Institute of Education, Nanjing 210013, Jiangsu Provinee, China高影响力机构 出　　处：《The Journal of Biomedical Research》索引2010年第24卷第3期,共11页高影响力期刊 摘　　要：Objective:MicroRNA (miRNA) expression is deregulated in many types of human cancers.We sought to investigate the expression patterns of the miRNAs,miR-21,miR-145 and miR-155 in sporadic gastric cancer in a Chinese population.Methods:Total RNA was extracted from archived gastric cancer tissues and adjacent non-cancerous tissues from 20 pairs of paraffin-embedded specimens.Expression levels of miR-21,miR- 145 and miR-155 were detected by quantitative reverse transcriptase PCR using a specific stem-loop primer,with U6 as the internal reference gene.Results:The expression of miR-21 and miR-155 in gastric cancer samples was significantly higher than in paired non-cancerous samples(P<0.05).There was no statistically significant difference in expression levels of miR-145 between cancerous and non-cancerous tissues(P>0.05).Conclusion:In Chinese sporadic gastric cancer tissues,the expressions of the oncogenic miR-21 and miR-155 were significantly up-regulated,while the expression of the tumor suppressor miR-145 was decreased,although this decrease was not statistically significant.Thus there is specificity in the miRNA expression pattern in gastric cancers in the Chinese population. 关 键 词：miRNAs 癌组织 胃癌 成熟 高架 患者 肿瘤抑制基因 表达模式
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