Expression Analysis of Proline Metabolism-related Genes From Halophyte Arabis stelleri under Osmotic Stress Conditions

查看全文 作　　者：Yuchul [1,2]Jung;Jungan [1,2]Park;Yunjung [1]Choi;Jin-Gweon [1]Yang;Donggiun [1]Kim;Beom-Gi [3]Kim;Kyunghee [3]Roh;Dong-Hee [4]Lee;Chung-Kyoon [5]Auh;Sukchan [1]Lee

机构地区：[1]Department of Genetic Engineering,Sungkyunkwan University;[2]Institute of Life Science and Technology,Sungkyunkwan University;[3]National Academy of Agricultural Science,Rural Development Administration;[4]Department of Life Science,Ewha Womans University;[5]Department of Biological Sciences,Mokpo National University

出　　处：《Journal of Integrative Plant Biology》索引2010年第52卷第10期,共13页

基　　金：supported by a grant from the Cooperative Research Projects for Bioenergy Crop Development RDA (RIMS20070201036026) 摘　　要：Arabis stelleri var.japonica evidenced stronger osmotic stress tolerance than Arabidopsis thaliana.Using an A.thaliana microarray chip,we determined changes in the expression of approximately 2 800 genes between A.stelleri plants treated with 0.2 M mannitol versus mock-treated plants.The most significant changes in the gene expression patterns were in genes defining cellular components or in genes associated with the endomembrane system,stimulus response,stress response,chemical stimulus response,and defense response.The expression patterns of three de novo proline biosynthesis enzymes were evaluated in A.stelleri var.japonica seedlings treated with 0.2 M mannitol,0.2 M sorbitol,and 0.2 M NaCl.The expression of 1-pyrroline-5-carboxylate synthetase was not affected by NaCl stress but was similarly induced by mannitol and sorbitol.The proline dehydrogenase gene,which is known to be repressed by dehydration stress and induced by free L-proline,was induced at an early stage by mannitol treatment,but the level of proline dehydrogenase was increased later by treatment with both mannitol and NaCl.The level of free L-proline accumulation increased progressively in response to treatments with mannitol,sorbitol,and NaCl.Mannitol induced L-proline accumulation more rapidly than NaCl or sorbitol.These findings demonstrate that the osmotic tolerance of the novel halophyte,Arabis stelleri,is associated with the accumulation of L-proline. 关键词：脯氨酸脱氢酶相关基因渗透胁迫盐生植物表达分析芥菜酸代谢基因表达模式

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Expression Analysis of Proline Metabolism-related Genes From Halophyte Arabis stelleri under Osmotic Stress Conditions

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