Single Nucleotide Polymorphism of FSHβ Gene Associated with Reproductive Traits in Japanese Flounder (Paralichthys olivaceus)

查看全文 作　　者：HE [1,2]Feng;WEN [2]Haishen;YU [1]Dahui;LI [2]Jifang;SHI [2]Bao;CHEN [2]Caifang;ZHANG [2]Jiaren;JIN [2]Guoxiong;CHEN [2]Xiaoyan;Shi [2]Dan;Yang [2]Yanping

机构地区：[1]South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, P. R. China;[2]Fisheries College, Ocean University of China, Qingdao 266003, P. R. China

出　　处：《Journal of Ocean University of China》索引2010年第9卷第4期,共4页

基　　金：supported by the open-fund of Key Laboratory of Fisheries Genetic Resources and Aquaculture,Chinese Academy of Fisheries Sciences of China (2008B1207);New Teacher Special Fund of Doctor of Ministry of Education of China (20090132120006) 摘　　要：Follicle stimulating hormone β (FSHβ) of Japanese flounder (Paralichthys olivaceus) plays a key role in the regulation of gonadal development.This study aimed to investigate molecular genetic characteristics of the FSHβ gene and elucidate the effects of single nucleotide polymorphisms (SNPs) of FSHβ on reproductive traits in Japanese flounder.We used polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) and sequencing of the FSHβ gene in 60 individuals.We identified only an SNP (T/C) in the coding region of exon3 of FSHβ.The SNP (T/C) did not lead to amino acid changes at the position 340 bp of FSHβ gene.Statistical analysis showed that the SNP was significantly associated with testosterone (T) level and gonadosomatic index (GSI) (P<0.05).Individuals with genotype TC of the SNP had significantly higher serum T levels and GSI (P<0.05) than that of genotype CC.Therefore, FSHβ gene could be a useful molecular marker in selection for prominent reproductive trait in Japanese Flounder. 关键词：单核苷酸多态性相关基因日本川鲽繁殖性状 FSH 牙鲆分子标记选择单链构象多态性

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Single Nucleotide Polymorphism of FSHβ Gene Associated with Reproductive Traits in Japanese Flounder (Paralichthys olivaceus)

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