Proteomic analysis of human epithelial ovarian cancer xenografts in immunodeficient mice exposed to chronic psychological stress

查看全文 作　　者：GAO [1]Jun;GAO [2]GuoLan;ZHANG [1]YanYu;WANG [1]Fen

机构地区：[1]Department of Gynecology and Obstetrics, the First Affiliated Hospital of Nanchang University, Nanchang 330006, China;[2]Department of Gynecology, Aviation General Hospital, Beijing 100012, China

出　　处：《Science China(Life Sciences)》索引2011年第54卷第2期,共9页

基　　金：supported by the National Natural Science Foundation of China (Grant Nos. 30860301 and 30660192) 摘　　要：This work aims at comparing alterations in the proteomes of human epithelial ovarian cancer xenografts between stressed and non-stressed immunodeficient mice as well as exploring the molecular mechanisms linking chronic psychological stress to ovarian cancer oncogenesis and progression.SK-OV-3 cells were injected subcutaneously into nude mice.The stress group was subjected to a chronic physical restraint protocol for 6 h on 35 consecutive days,while the control group was unrestrained.All mice were sacrificed on day 36 after SK-OV-3 cell injection,and tumors were excised.Tumor tissues were processed for 2D gel electrophoresis,mass spectrometry(nanoUPLC-ESI-MS/MS) and Western blotting.The expression of 20 proteins was found to be significantly altered between the stress and control groups,of which 14 were up-regulated,five were down-regulated,and one protein was found only in the stress group.All proteins were identified by UPLC-ESI-MS/MS,and Western blotting results were consistent with those of proteomic methods.The present results provide new evidence relating to the molecular mechanism underlying the relationship between psychological stress and tumor progression. 关键词：心理应激蛋白质组免疫缺陷卵巢癌小鼠慢性 WESTERN印迹上皮

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Proteomic analysis of human epithelial ovarian cancer xenografts in immunodeficient mice exposed to chronic psychological stress

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