Metastasis-associated protein 1 induces VEGF-C and facilitates lymphangiogenesis in colorectal cancer

查看全文 作　　者：Bin [1]Du;Zhen-Yu [1]Yang;Xue-Yun [1]Zhon;Mao [1]Fang;Yong-Rong [1]Yan;Guo-Long [2]Qi;Yun-Long [3]Pan;Xu-Long [1]Zhou

机构地区：[1]Department of Pathology, MedicalSchool of Jinan University, Guangzhou 510632, GuangdongProvince, China;[2]Division of Medical Inforrnatics, Medical Schoolof Jinan University, Guangzhou 510632, Guangdong Province,China;[3]Department of General Surgery, First AffiliatedHospital of Jinan l~lniversity, Guangzhou 510632, GuangdongProvince, China

出　　处：《World Journal of Gastroenterology》索引2011年第17卷第9期,共8页

基　　金：Supported by National Natural Science Foundation of China(No.30772131);Fundamental Research Funds for Central Universities(No.21609313)from Ministry of Education of China 摘　　要：AIM:To study the correlation between high metastasisassociated protein 1(MTA1)expression and lymphangiogenesis in colorectal cancer(CRC)and its role in production of vascular endothelial growth factor-C(VEGF-C). METHODS:Impact of high MTA1 and VEGF-C expression levels on disease progression and lymphovasculardensity(LVD,D2-40-immunolabeled)in 81 cases of human CRC was evaluated by immunohistochemistry. VEGF-C mRNA and protein expressions in human LoVo and HCT116 cell lines were detected by real-time polymerase chain reaction and Western blotting,respectively,with a stable expression vector or siRNA. RESULTS:The elevated MTA1 and VEGF-C expression levels were correlated with lymph node metastasis and Dukes stages(P<0.05).Additionally,high MTA1 expression level was correlated with a large tumor size(P< 0.05).A significant correlation was found between MTA1 and VEGF-C protein expressions in tumor cells(r=0.371, P<0.05).Similar to the VEGF-C expression level,high MTA1 expression level was correlated with high LVD in CRC(P<0.05).Furthermore,over-expression of MTA1 significantly enhanced the VEGF-C mRNA and protein expression levels,whereas siRNAs-knocked down MTA1 decreased the VEGF-C expression level. CONCLUSION:MTA1,as a regulator of tumor-associated lymphangiogenesis,promotes lymphangiogenesis in CRC by mediating the VEGF-C expression. 关键词：VEGF 相关蛋白淋巴管大肠癌血管内皮生长因子免疫组织化学方法 siRNA 肿瘤细胞

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Metastasis-associated protein 1 induces VEGF-C and facilitates lymphangiogenesis in colorectal cancer

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