In vitro morphogenic response of leaf sheath of Phyllostachys bambusoides

查看全文 作　　者：Yurika [1]H.Komatsu;Katherine Derlene Batagin-[2]Piotto;Gilvano Ebling [1]Brondani;Antnio Natal Gon[2,1]alves;Marcílio de [2]Almeida

机构地区：[1]Graduate Program in Forest Resources,Department of Forest Sciences,'Luiz de Queiroz' College of Agriculture,University of So Paulo(ESALQ/USP),Avenida Pádua Dias,11,Piracicaba,So Paulo,Brazil;[2]Graduate Program in Biochemistry of Plants,Department of Biological Sciences,'Luiz de Queiroz' College of Agriculture,University of So Paulo(ESALQ/USP),Avenida Pádua Dias,11,Piracicaba,So Paulo

出　　处：《Journal of Forestry Research》索引2011年第22卷第2期,共7页

基　　金：supported by FAPESP (So Paulo Research Foundation, Brazil);CAPES (Coordination for the Improvement of Higher Level, Brazil) 摘　　要：Nodal segments from secondary branches of saplings of Phyllostachys bambusoides were inoculated in MS medium to assess the in vitro morphogenic response of leaf sheath through the induction to callogenesis by Picloram(4-amino-3,5,6-trichloropicolinic acid) at different concentrations of carbohydrate under the same conditions with presence or absence of luminosity.In our experiment,secondary explants were kept in MS medium containing 8.0 mg·L-1 of Picloram for the callus formation.Calluses were transferred in MS medium supplemented with sucrose,fructose and glucose(control,2%,4% and 6%).Results show that Picloram induced the callogenesis in leaf sheath.The secondary embryogenesis was formed in yellow-globular callus.The sucrose as carbohydrate source in the absence of light was more efficient to induce rhizogenesis.Glucose was more efficiency in the presence of light.Callogenic induction and further embryogenesis evidenced the competence and determination of leaf sheath cells. 关键词：形态发生反应体外毛竹 MS培养基碳水化合物胚胎发育愈伤组织

相关文献

参考文献（58）
引证文献（3）
耦合文献（8）

参考文献(58)

引证文献(3)

耦合文献(8)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

In vitro morphogenic response of leaf sheath of Phyllostachys bambusoides

参考文献(58)

引证文献(3)

耦合文献(8)

用户提示