Effect of chitooligosaccharides on cyclin D1,bcl-xl and bcl-2 mRNA expression in A549 cells using quantitative PCR

查看全文 作　　者：LI [1]Xian;WANG [2]Ju;CHEN [2]XiaoJia;TIAN [1]JinHuan;LI [1]LiHua;ZHAO [1]MingYan;JIAO [1]YanPeng;ZHOU [1]ChangRen

机构地区：[1]Department of Materials Science and Engineering, Jinan University, Guangzhou 510632, China1;[2]Bioengineering Institute, Jinan University, Guangzhou 510632, China

出　　处：《Chinese Science Bulletin》索引2011年第56卷第15期,共4页

基　　金：supported by the National High Technology Research and Development Program of China (2007AA091603) 摘　　要：Chitooligosaccharide (COS) is derived from the chemical and enzymatic hydrolysis of chitosan and has been reported to have potent antitumor activity.Our study investigated the effects of five chitooligomers ranging from the dimer form to the hexamer form (chitobiose,chitotriose,chitotetraose,chitopentaose,chitohexaose) on the expression of cyclin D1,bcl-2 and bcl-xl mRNA in A549 cells using reverse transcription quantitative real-time PCR.We demonstrated that,of the five chitooligomers used,chitohexaose (COS6) had the most potent inhibitory effect on A549 cell proliferation.COS6 also significantly down regulated cyclin D1 and bcl-xl mRNA expression levels.Our data suggested that COS6 exerts its antitumor activity by two different mechanisms:(1) COS6-mediated inhibition of Cyclin D1 levels leads to suppression of tumor cell proliferation;and (2) COS6-mediated down-regulation of the pro-survival protein,Bcl-xl,promotes the apoptosis of tumor cells. 关键词：细胞周期蛋白D1 MRNA表达水平 A549细胞壳寡糖定量PCR 肿瘤细胞凋亡抗肿瘤活性 CYCLIN

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Effect of chitooligosaccharides on cyclin D1,bcl-xl and bcl-2 mRNA expression in A549 cells using quantitative PCR

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