Cloning of vacuolar H^+ -ATPase subunit c genes from Japanese iris, and functional characterization in yeast

查看全文 作　　者：ZHOU Ai-[1]min;WU [1]Duo;CHE Dai-[1]di;WANG Jin-[1,3]gang;LIU Shen-[2]kui;YANG Chuan-[3]ping

机构地区：[1]Horticulture college, Northeast Agricultural University, Harbin, 150030, China.;[2]Alkali Soil Natural Environmental Science Center (ASNESC), NortheastForestry University, Harbin 150040 China;[3]Key Laboratory of Forest Tree Genetic Improvement and Biotechnology,Ministry of Education, Northeast Forestry University, Harbin, 150040,China

出　　处：《Journal of Forestry Research》索引2011年第22卷第3期,共6页

基　　金：supported by the Heilongjiang Province Postdoctoral Science Foundation(LBH-Q10144) 摘　　要：Five different isoforms (IrlVHA-c1-c5) of V-ATPase subunit c (VHA-c) were cloned from a Japanese iris (Iris lactea Pall. var. chinensis Fisch. Koidz) cDNA library using degenerate primers PCR and the 5'-RACE technique. The sequence analysis showed the open reading frame (ORF) of the IrlVHA-c1 c5 to be 495 bp, corresponding to a protein of 164 amino acids. Among the five isoforms, IrlVHA-c1 and IrlVHA-c2 are completely homologous. The IrlVHA-c protein is localized at the vacuolar membrane as indicated by a green fluorescent protein (GFP) marker. Its over-expression in yeast could enhance yeast tolerance to NaCl stress. These results show that there are at least five genes encoding different isoforms of IrlVHA-c in Japanese iris and IrlVHA-c is important for the function of V-ATPase. 关键词：ATP酶液泡膜虹膜日本功能特性酵母克隆亚基

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Cloning of vacuolar H^+ -ATPase subunit c genes from Japanese iris, and functional characterization in yeast

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