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Characterization of small RNAs from Ulva prolifera by high-throughput sequencing and bioinformatics analysis

查看全文 作  者:HUANG [1,2]AiYou;WANG [1]GuangCe;HE [1,2]LinWen;NIU [1]JianFeng;ZHANG [1]BaoYu 高影响力作者 机构地区:[1]Institute of Oceanology, the Chinese Academy of Sciences (IOCAS), Qingdao 266071, China;[2]Graduate University of the Chinese Academy of Sciences, Beijing 100049, China高影响力机构 出  处:《Chinese Science Bulletin》索引2011年第56卷第27期,共6页高影响力期刊 基  金:supported by the Scientific and Technical Supporting Programs of China (2008BAC49B01);the National Natural Science Foundation of China (30830015) 摘  要:The seaweed Ulva prolifera exists in 2 different states; attached to rocks or free-floating. However, there is little difference between the structures of the 2 states. U. prolifera thalli show significant differences in growth rate, with the attached thalli growing at a normal rate and free-floating thalli growing at a much faster rate. This raised the possibility that the growth of the two states may be regulated differently. miRNAs are important post-transcriptional regulators. In higher plants and animals, miRNAs have been extensively studied but they have been rarely studied in algae. To identify U. prolifera miRNAs and to investigate their possible roles in proliferation, we constructed and sequenced small RNA (sRNA) libraries from U. prolifera. Our results show that U. prolifera has a complex small RNA system that might play important roles in various processes. 关 键 词:小分子RNA 生物信息学 增殖 分析表征 石莼 测序 高通量 MIRNA
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