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Distribution and relative activity of matrix metalloproteinase-2 in human coronal dentin

查看全文 作　　者：Lee W. [1]Boushell;Masaru [2]Kaku;Yoshiyuki [3]Mochida;Mitsuo [4]Yamauchi 高影响力作者 机构地区：[1]Department of Operative Dentistry, School of Dentistry, University of North Carolina;[2]Department of Bio-Prosthodontics, Medical and Dental Science, Niigata University Graduate School Chuo-ku;[3]Department of Periodontology and Oral Biology, Division of Oral Biology, Boston University, Henry M. Goldman School of Dental Medicine, Center for Advanced Biomedical Research;[4]Dental Research Center, School of Dentistry, University of North Carolina高影响力机构 出　　处：《International Journal of Oral Science》索引2011年第3卷第4期,共8页高影响力期刊 摘　　要：The presence of matrix metalloproteinase-2 (MMP-2) in dentin has been reported, but its distribution and activity level in mature human coronal dentin are not well understood. The purpose of this study was to determine the MMP-2 distribution and relative activity in demineralized dentin. Crowns of twenty eight human molars were sectioned into inner (ID), middle (MD), and outer dentin (OD) regions and demineralized. MMP-2 was extracted with 0.33 mol·L -1 EDTA/2 mol·L -1 guanidine-HCl, pH 7.4, and MMP-2 concentration was estimated with enzyme-linked immunoabsorbant assay (ELISA). Further characterization was accomplished by Western blotting analysis and gelatin zymography. The mean concentrations of MMP-2 per mg dentin protein in the dentin regions were significantly different (P=0.043): 0.9 ng (ID), 0.4 ng (MD), and 2.2 ng (OD), respectively. The pattern of MMP-2 concentration was OD>ID>MD. Western blotting analysis detected ~66 and ~72 kDa immunopositive proteins corresponding to pro-and mature MMP-2, respectively, in the ID and MD, and a ~66 kDa protein in the OD. Gelatinolytic activity consistent with MMP-2 was detected in all regions. Interestingly, the pattern of levels of Western blot immunodetection and gelatinolytic activity was MD>ID>OD. The concentration of MMP-2 in human coronal dentin was highest in the region of dentin that contains the dentinoenamel junction and least in the middle region of dentin. However, levels of Western blot immunodetection and gelatinolytic activity did not correlate with the estimated regional concentrations of MMP-2, potentially indicating region specific protein interactions. 关 键 词：基质金属蛋白酶-2 牙本质 相对活性 人类 免疫印迹分析 WESTERN印迹 平均浓度 免疫检测
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