Systemic PCD occurs in TMV-tomato interaction

查看全文 作　　者：ZHOU ShuMin, LIU WenNa, KONG LinAn & WANG Mao College of Biological Sciences, China Agriculture University, Beijing 100094, China

出　　处：《Science China(Life Sciences)》索引2008年第51卷第11期,共11页

基　　金：the National Natural Science Foundation of China (Grant No. 30370084) 摘　　要：In hypersensitive response (HR), programmed cell death (PCD) is reported as a powerful defense mechanism in plant immune responses to pathogen. However, little is known about the PCD in sys-temic acquired resistance (SAR). Using tobacco mosaic virus (TMV) to infect the tomato (Lycopersicon esculentum cv. Jiafen 16) we found that localized TMV-infection could induce cell death in the uninoculated parts of the tomatoes, where the enzyme-linked immunosorbent assay (ELISA) showed no spreading virus. The biological and molecular characterization of this cell death was shown as fol-lowing: chromatin condensed and formed peripheral conglomeration in nuclei; cell nucleus were TUNEL positive labeled; genomic DNA was fragmented and showed DNA laddering; mitochondria and chloroplast were disrupted; tonoplast and plasma membrane were shrunk and degradated. These re-sults suggested that with an absence of TMV spread, the local TMV-infection on certain tomato leaves could induce systemic PCD in the root-tips, stem-apices and uninoculated leaves. The systemic PCD has various initiation and synchronization in such tissues and is distinct in inducement and exhibition from HR-PCD and SAR. 关键词：programmed cell death SYSTEMIC PCD TUNEL assay DNA LADDER TMV TOMATO

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Systemic PCD occurs in TMV-tomato interaction

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