Cultivation of human liver cell lines with microcarriers acting as biological materials of bioartificial liver

查看全文 作　　者：Gao [1]Y;Xu [1]XP;Hu [1]HZ;Yang [1]JZ 机构地区：[1]Mil Med Univ 1st, Dept Gen Surg, Zhujiang Hosp, Guangzhou 510282, Guangdong Prov, Peoples R China 出　　处：《World Journal of Gastroenterology》索引1999年第5卷第3期,共4页 基　　金：Supported by the National Natural Science Foundation of China,No.39570212. 摘　　要：AIM To improve the Cultivation efficiency and yield of human liver cell line CL--l.METHODS High--density cultivation of CL--l onmicrocarriers was carried out with periodicobservation of their growth and proliferation.The specific functions of human liver cell werealso determined.RESULTS Cells of CL-1 cell line grew well onmicrocarrier Cytodex--3 and on the 7th day thepeak was reached. The amount of CLI cells was2.13 x 10’ and the total amount of albuminsynthesis reached 71 .23 ig, urea synthesis23 .32 mg and diazepam transformation 619 .7 ugrespectively. The yield of CL-l on microcarrierswas 49.3 times that of conventional cultivation.The amounts of albumin synthesis, ureasynthesis and diazepam transformation were39.8 times, 41 .6 times and 33.3 times those ofconventional Cultivation, respectively.CONCLUSION The human liver cell line CL--1 canbe Cultivated to a high density with Cytodex--3and has better biological functions. High-densityCultivation of CLI on microcarriers can act asthe biological material of bioartificial liver. 关 键 词：CELL FIGURE