Helicobacter species and gut bacterial DNA in Meckel's diverticulum and the appendix

查看全文 作　　者：Peren H [1]Karagin;Unne [2]Stenram;Torkel Wadstr[1]m;sa [1]Ljungh

机构地区：[1]Department of Medical Microbiology,Lund University,S lvegatan 23,SE-223 62 Lund,Sweden;[2]Department of Pathology,Lund University,SE-22185 Lund,Sweden

出　　处：《World Journal of Gastroenterology》索引2011年第17卷第36期,共5页

基　　金：Supported by A grant from the University Hospital of Lund(ALF) to Torkel Wadstrm and a grant from the John;Forssman's foundation,the Royal Physiographic Society in Lund to Peren Karagin 摘　　要：AIM:To analyse the possible association of various Helicobacter species and certain common gut bacteria in patients with Meckel's diverticulum and appendicitis.METHODS:A nested-polymerase chain reaction (PCR),specific to 16S rRNA of the Helicobacter genus,was performed on paraffin embedded samples,50 with acute appendicitis,50 normal appendixes,and 33 Meckel's diverticulum with gastric heterotopia and/or ulcer.Helicobacter genus positive samples were sequenced for species identification.All samples were also analysed for certain gut bacteria by PCR.RESULTS:Helicobacter pullorum DNA was found in one out of 33 cases and Enterobacteria in two cases of Meckel's diverticulum.Helicobacter pylori (H.pylori) was found in three,Enterobacter in 18,and Bacteroides in 19 out of 100 appendix samples by PCR.Enterococcus was not found in any MD or appendix samples.All H.pylori positive cases were from normal appendixes.CONCLUSION:Helicobacter is not an etiological agent in the pathogenesis of symptomatic Meckel's diverticulum or in acute appendicitis. 关键词：幽门螺杆菌肠道细菌 DNA 巢式聚合酶链反应菌种 RRNA基因物种鉴定 PCR

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Helicobacter species and gut bacterial DNA in Meckel's diverticulum and the appendix

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