Hepatic steatosis prevents heme oxygenase-1 induction by isoflurane in the rat liver

查看全文 作　　者：Patrick [1]Stoll;Christian I [1]Schwer;Ulrich [1]Goebel;Hartmut [1]Buerkle;Alexander [1]Hoetzel;Rene [1]Schmidt 机构地区：[1]Department of Anesthesiology and Critical Care Medicine,Freiburg University Medical Center 出　　处：《World Journal of Gastroenterology》索引2011年第17卷第37期,共7页 基　　金：Supported by Departmental funding 摘　　要：AIM:To characterize the inductive effects of isoflurane(ISO) on hepatic heme oxygenase-1(HO-1) in an animal model of hepatic steatosis.METHODS:Lean(LEAN) and obese(FAT) Zucker rats were randomized into 4 groups:1:LEAN + pentobarbital sodium(PEN);2:LEAN + ISO;3:FAT + PEN;4:FAT + ISO.The animals were mechanically ventilated for 6 h.In vitro analyses of liver tissue included determination of HO-1 mRNA and protein expression as well as measurement of HO enzyme activity and immunohistochemical analyses.RESULTS:Compared to PEN treatment,ISO administration profoundly induced hepatic HO-1 mRNA and protein expression and significantly increased HO enzyme activity in lean Zucker rats.In contrast,no difference in HO-1 gene expression was observed after ISO or PEN anesthesia in obese Zucker rats.CONCLUSION:The present study demonstrates that ISO is an inducer of hepatic HO-1 gene expression in non-steatotic organs but failed to upregulate HO-1 in steatotic livers. 关 键 词：血红素加氧酶 大鼠肝脏 诱导效应 脂肪肝 异氟醚 动物模型 精益生产 蛋白表达